CEBPD anticorps (N-Term)

N° du produit ABIN233849

Détail du produit anti-CEBPD anticorps

Antigène: CEBPD (CCAAT/enhancer Binding Protein (C/EBP), delta (CEBPD))

Épitope: N-Term

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CEBPD est non-conjugé

Application: Western Blotting (WB), ELISA

Specificité: This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific for mouse C/EBP delta protein. A BLAST analysis was used to suggest cross-reactivity with C/EBPd from human sources based on 100% homology with the immunizing sequence. No cross-reactivity is observed with mouse C/EBPa or C/EBPb. Reactivity with C/EBPd from other sources has not been determined.

Réactivité croisée: Humain

Attributs du produit: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. C/EBP proteins are DNA-binding proteins that are important transcriptional activators in the regulation of genes involved in immune and inflammatory responses and may play an important role in the regulation of the several genes associated with activation and/or differentiation of macrophages. Members of the C/EBP transcription factor family are related by a high degree of amino acid sequence identity to the basic leucine zipper DNA-binding domain and show distinct but overlapping patterns of tissue- and stage-restricted expression. Osada et al. identified the consensus binding site for the family as RTTGCGYAAY (R = A or G, and Y = C or T). Phosphorylation of C/EBPd may increase binding activity, whereas phosphorylation of the a and b family members may decrease binding affinity. C/EBPd is a nuclear protein that binds DNA as a dimer and can form stable heterodimers with all other C/EBP family members.

Purification: purified

Stérilité: Sterile filtered

Immunogène: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to the amino terminus of mouse C/EBP.
Immunogen Type: RecombinantProtein

Isotype: IgG

Information d'application

Indications d'application: This affinity purified antibody has been tested for use in ELISA and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 35 kDa in size corresponding to C/EBPd by western blotting in the appropriate cell lysate or extract.   The predicted molecular weight of mouse C/EBPd is 28.8 kDa. This antibody is capable of detecting both over-expressed and endogenous C/EBPd.

Commentaires:

Gene Name: CEBPD

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.83 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Expiration date is three (3) months from date of opening.

Date de péremption: 3 months

Références pour anticorps anti-CEBPD (ABIN233849)

Puzio-Kuter, Laddha, Castillo-Martin, Sun, Cordon-Cardo, Chan, Levine: "Involvement of tumor suppressors PTEN and p53 in the formation of multiple subtypes of liposarcoma." dans: Cell death and differentiation, Vol. 22, Issue 11, pp. 1785-91, (2015) (PubMed).

Sarkar, Sharan, Wang, Pawar, Cantwell, Johnson, Morrison, Wang, Sterneck: "Identification of a Src tyrosine kinase/SIAH2 E3 ubiquitin ligase pathway that regulates C/EBPδ expression and contributes to transformation of breast tumor cells." dans: Molecular and cellular biology, Vol. 32, Issue 2, pp. 320-32, (2012) (PubMed).

Balamurugan, Wang, Tsai, Sharan, Anver, Leighty, Sterneck: "The tumour suppressor C/EBPδ inhibits FBXW7 expression and promotes mammary tumour metastasis." dans: The EMBO journal, Vol. 29, Issue 24, pp. 4106-17, (2011) (PubMed).

Détails sur CEBPD

Antigène: CEBPD (CCAAT/enhancer Binding Protein (C/EBP), delta (CEBPD))

Autre désignation: CEBP Delta (CEBPD Produits)

Synonymes: anticorps C/EBP-delta, anticorps CELF, anticorps CRP3, anticorps NF-IL6-beta, anticorps C/EBPd, anticorps LOC398729, anticorps LOC398730, anticorps MGC114740, anticorps c/ebp-delta, anticorps celf, anticorps crp3, anticorps nf-il6-beta, anticorps c/ebpd, anticorps wu:fa99c04, anticorps wu:fb61h03, anticorps CEBPD, anticorps cebpd, anticorps CCAAT/enhancer binding protein (C/EBP), delta, anticorps CCAAT/enhancer binding protein delta, anticorps CCAAT/enhancer binding protein delta L homeolog, anticorps CCAAT/enhancer binding protein delta S homeolog, anticorps Cebpd, anticorps CEBPD, anticorps cebpd.L, anticorps cebpd.S, anticorps cebpd

Sujet: This antibody is designed, produced, and is suitable for Cancer, Immunology and Nuclear Signaling research. C/EBP proteins are DNA-binding proteins that are important transcriptional activators in the regulation of genes involved in immune and inflammatory responses and may play an important role in the regulation of the several genes associated with activation and/or differentiation of macrophages.  Members of the C/EBP transcription factor family are related by a high degree of amino acid sequence identity to the basic leucine zipper DNA-binding domain and show distinct but overlapping patterns of tissue- and stage-restricted expression.  Osada et al. identified the consensus binding site for the family as RTTGCGYAAY (R = A or G, and Y = C or T).  Phosphorylation of C/EBPd may increase binding activity, whereas phosphorylation of the a and b family members may decrease binding affinity.  C/EBPd is a nuclear protein that binds DNA as a dimer and can form stable heterodimers with all other C/EBP family members.
Synonyms: C/EBP related protein 3 antibody, CCAAT/enhancer binding protein delta antibody, CEBP D antibody, CEBPD antibody, CELF antibody, Crp 3 antibody, Crp3 antibody, NF IL6 beta antibody, Nuclear factor NF IL6 beta antibody

ID gène: 12609, 110347410

UniProt: Q00322