The molecular weight of the recognized antigen is 150kDa. CD49d antigen is the integrin alpha-4 chain non covalently associated with the integrin beta-1-chain (CD29 molecule) forming the VLA4 complex. Reacts with monocytes, T cells, B cells, thymocytes and Langerhans cells.
Flow cytometry Basic studies of VLA4 mediated adhesion and its interaction with the VCAM-1 structure. Fluorescent microscopy or flow cytometry: 2 µg/5x105 cells/test Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Reconstitute with 1 mL distilled water.
Buffer
Lyophilized from PBS containing 1 mg/mL BSA.
Agent conservateur
Without preservative
Conseil sur la manipulation
Avoid multiple freeze/thaw cycles. The addition of 0.1 % (w/v) sodium azide is recommended for storage up to one month at 2-8 °C. Centrifuge product if not completely clear after standing at room temperature. Prepare working dilution only prior to immediate use.
Stock
4 °C/-20 °C
Stockage commentaire
Store lyophilized product at 2-8 °C. After reconstitution, aliquot and store at -28 °C.