LAT2 anticorps (AA 91-243)
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- Antigène Voir toutes LAT2 Anticorps
- LAT2 (Linker For Activation of T Cells Family, Member 2 (LAT2))
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Épitope
- AA 91-243
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Reactivité
- Humain
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp LAT2 est non-conjugé
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Specificité
- The polyclonal antibody recognizes the defined epitope (aa 91-243) of human Non-T cell Activation Linker (NTAL), also known as LAB (linker of activated B cells), a 30 kDa transmembrane adaptor protein present in membrane microdomains (rafts) of B cells, NK cells and myeloid cells.
- Purification
- Protein-A affinity chromatography.
- Immunogène
- Recombinant cytoplasmic domain (aa 91-243) of human NTAL.
- Top Product
- Discover our top product LAT2 Anticorps primaire
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- Indications d'application
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Suitable for Western blot under Non-Reducing conditions: 15 % separating SDS-PAGE gel. Recommended dilution: 1 μg/mL, incubation 60 min in RT, or overnight at 4 °C. Positive control: RAMOS human lymphoma cell line. THP-1 human monocytic leukemia cell line. Negative control: JURKAT human T cell leukemia cell line. Sample preparation: Resuspend approx.50 mil. cells in 1 mLcold Lysis buffer (1 %laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Proteaseinhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysatewith non-reducing SDS-PAGE sample buffer. Immunohistochemistry on paraffin sections: 10 μg/mL. Positive tissue: spleen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- PBS, pH 7.4 with 15 mM sodium azide as preservative.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Avoid repeated freezing and thawing.
- Stock
- 4 °C/-20 °C
- Stockage commentaire
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Antigène
- LAT2 (Linker For Activation of T Cells Family, Member 2 (LAT2))
- Autre désignation
- LAT2 / WBSCR15 (LAT2 Produits)
- Synonymes
- anticorps LAB, anticorps NTAL, anticorps WBSCR15, anticorps WBSCR5, anticorps WSCR5, anticorps LAT2, anticorps MGC139435, anticorps Ntal, anticorps Wbscr5, anticorps AW125574, anticorps Wbscr15, anticorps linker for activation of T-cells family member 2, anticorps linker for activation of T cells family, member 2, anticorps LAT2, anticorps Lat2
- Sujet
- NTAL (Non-T cell activation linker), also known as LAB (Linker for activation of B cells), is a 30 kDa double-palmitoylated transmembrane adaptor protein expressed by B cells, NK cells, mast cells and macrophages. It is a negative regulator of early stages of BCR-dependent B cell signaling and serves as a negative regulator also in mast cells. However, in mast cells, NTAL also contributes to some activation processes, partially overlapping with LAT function.Synonyms: HSPC046, LAB, LLinker for activation of B-cells, Linker for activation of T-cells family member 2, Membrane-associated adapter molecule, NTAL, Non-T-cell activation linker, WBS15, WBSCR5, Williams-Beuren syndrome chromosomal region 15 protein
- ID gène
- 7462
- NCBI Accession
- NP_054865
- UniProt
- Q9GZY6
- Pathways
- Fc-epsilon Receptor Signaling Pathway, BCR Signaling
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