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5-Methylcytosine anticorps (Biotin)

IF, ICC Hôte: Souris Monoclonal 5MC-CD Biotin
N° du produit ABIN2451913
  • Antigène
    5-Methylcytosine
    Reactivité
    Veuillez nous consulter SVP
    Hôte
    • 22
    • 8
    • 3
    Souris
    Clonalité
    • 24
    • 9
    Monoclonal
    Conjugué
    • 17
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Biotin
    Application
    • 20
    • 16
    • 13
    • 10
    • 8
    • 8
    • 7
    • 5
    • 5
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Immunofluorescence (IF), Immunocytochemistry (ICC)
    Purification
    Purified
    Stérilité
    Sterile filtered
    Immunogène
    5-Methylcytosine conjugated to bovine serum albumin
    Clone
    5MC-CD
    Isotype
    IgM
  • Indications d'application
    1) Immunocytochemistry: ~50-100 fold dilution
    2) Immuno-blotting detection of DNA with 5-methylcytosine on nitrocellulose: ~1000 fold dilution
    Not tested with other application
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS with 50 % glycerol. Azide and carrier-protein free.
    Agent conservateur
    Azide free
    Stock
    -20 °C
    Stockage commentaire
    Upon receipt aliquot and store at -20 C
  • Sharif, Muto, Takebayashi, Suetake, Iwamatsu, Endo, Shinga, Mizutani-Koseki, Toyoda, Okamura, Tajima, Mitsuya, Okano, Koseki: "The SRA protein Np95 mediates epigenetic inheritance by recruiting Dnmt1 to methylated DNA." dans: Nature, Vol. 450, Issue 7171, pp. 908-12, (2007) (PubMed).

    Nishiyama, Ito, Yamaguchi, Koizumi, Sano: "A chloroplast-resident DNA methyltransferase is responsible for hypermethylation of chloroplast genes in Chlamydomonas maternal gametes." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 9, pp. 5925-30, (2002) (PubMed).

    Sano, Imokawa, Sager: "Detection of heavy methylation in human repetitive DNA subsets by a monoclonal antibody against 5-methylcytosine." dans: Biochimica et biophysica acta, Vol. 951, Issue 1, pp. 157-65, (1988) (PubMed).

    Sano, Royer, Sager: "Identification of 5-methylcytosine in DNA fragments immobilized on nitrocellulose paper." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 77, Issue 6, pp. 3581-5, (1980) (PubMed).

  • Antigène
    5-Methylcytosine
    Autre désignation
    5 Methylcytosine
    Classe de substances
    Chemical
    Sujet
    DNA methylation is a type of chemical modification of DNA that can be inherited and subsequently removed without changing the original DNA sequence. Therefore it is part of the epigenetic code and is also the most well characterized epigenetic mechanism. DNA methylation results in addition of a methyl group to DNA - for example, to the number 5 carbon of the cytosine pyrimidine ring - which involves reduction in gene expression. In adult somatic tissues, DNA methylation typically occurs in a CpG dinucleotide context, non-CpG methylation is prevalent in embryonic stem cells. This hybridoma has been constructed by Prof. H. Sano.
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