HIV-1 p24
Reactivité: Human Immunodeficiency Virus (HIV)
ELISA
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
1. Western blot: 1/2,000~1/5000 2. Dot blot (assay depemdemt 3. Immunoprecipitation (assay dependent) 4. ELISA (assay dependent) Other applications have not been tested.
Restrictions
For Research Use only
Format
Liquid
Buffer
0.09 % sodium azide
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
-20 °C
Stockage commentaire
Upon receipt aliquot and store at -20 C
Freed: "HIV-1 gag proteins: diverse functions in the virus life cycle." dans: Virology, Vol. 251, Issue 1, pp. 1-15, (1998) (PubMed).
Saito, Morimoto, Ohara, Takamizawa, Nakata, Shinagawa: "Overproduction, purification, and diagnostic use of the recombinant HIV-1 Gag proteins, the precursor protein p55 and the processed products p17, p24, and p15." dans: Microbiology and immunology, Vol. 39, Issue 7, pp. 473-83, (1996) (PubMed).
Adachi, Gendelman, Koenig, Folks, Willey, Rabson, Martin: "Production of acquired immunodeficiency syndrome-associated retrovirus in human and nonhuman cells transfected with an infectious molecular clone." dans: Journal of virology, Vol. 59, Issue 2, pp. 284-91, (1986) (PubMed).
HIV-1 Gag p24 is a capsid protein that constitutes the core of AIDS virus HIV-1 and is produced by the digestion of its precursor Gag p55 by HIV-1 protease. This protein is indispensable to the reproduction of AIDS virus and constitutes an essential element for the AIDS virus particle construction. As this protein is detectable from the early stage of AIDS virus infection, it is used as a marker for observation of the patient's condition after treatment, as it indicates the amount of virus in the blood. Using this antiserum in Western blotting, the bands of 24 kD, 55 kD and 41 kD corresponding respectively to HIV-p24 and its precursors p55 and p41 were observed in the extract of the AIDS virus infected cells.