Rad6 (full length) anticorps

N° du produit ABIN2452101

Détail du produit

Antigène: Rad6

Épitope: full length

Reactivité: Humain, Souris, Rat, Hamster

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Réactivité croisée (Details): Not tested in other species.

Purification: Affinity purified with the immunogen conjugated to agarose

Stérilité: Sterile filtered

Immunogène: Human Rad6B full-length recombinant protein

Information d'application

Indications d'application: 1) Western blotting: 1,000~2,000 fold dilution
2) Immunoprecipitation (assay dependent)
3) Immunofluoscent staining (assay dependent)
Other applications have not been tested

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS (x1), 50 % glycerol. Azide and carrier free.

Agent conservateur: Azide free

Stock: -20 °C

Stockage commentaire: Upon arrival centrifuge briefly and store at -20 C.

Référence

Masuyama, Tateishi, Yomogida, Nishimune, Suzuki, Sakuraba, Inoue, Ogawa, Yamaizumi: "Regulated expression and dynamic changes in subnuclear localization of mammalian Rad18 under normal and genotoxic conditions." dans: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 10, Issue 8, pp. 753-62, (2005) (PubMed).

Détail du antigène

Antigène: Rad6

Sujet: Resume of DNA synthesis, which was halted by various physical and chemical factors, was triggered by repair system including Rad6. Rad6 (152a.a, 17.3 kDa) is the ubiqutin-conjugated enzyme E2A, and complex with two Rad18 Molecules, ubiquitin ligase E3, to ubiquitinate PCNA by utilizing poly-ubiquitin chain that HLTF-UBC13 synthesized and they are involved in translesion DNA synthesis (TLS) and template switch (TS). Two isoforms of Rad6, Rad6A and Rad6B differs in only 6 amino acids. They also play an important role in epigenetic transcriptional regulation by catalyzing the monoubiquitination of histone H2B at Lys-120.

UniProt: P49459, P63146