Shugoshin anticorps

N° du produit ABIN2452123

Détail du produit anti-Shugoshin anticorps

Antigène: Shugoshin (SGOL1) (Shugoshin 1 (SGOL1))

Reactivité: Schizosaccharomyces pombe

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Shugoshin est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Purification: Affinity purified

Stérilité: Sterile filtered

Information d'application

Indications d'application: 1) Western blotting: 1,000 fold dilution
2) Immunofluoresece staining: 100 fold dilution

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 1 mg/mL BSA, PBS ( pH 7.2), 50 % glycerol

Agent conservateur: Azide free

Stock: -20 °C

Références pour anticorps anti-Shugoshin (ABIN2452123)

Yamagishi, Sakuno, Shimura, Watanabe: "Heterochromatin links to centromeric protection by recruiting shugoshin." dans: Nature, Vol. 455, Issue 7210, pp. 251-5, (2008) (PubMed).

Watanabe, Kitajima: "Shugoshin protects cohesin complexes at centromeres." dans: Philosophical transactions of the Royal Society of London. Series B, Biological sciences, Vol. 360, Issue 1455, pp. 515-21, discussion 521, (2005) (PubMed).

Kitajima, Kawashima, Watanabe: "The conserved kinetochore protein shugoshin protects centromeric cohesion during meiosis." dans: Nature, Vol. 427, Issue 6974, pp. 510-7, (2004) (PubMed).

Détails sur Shugoshin

Antigène: Shugoshin (SGOL1) (Shugoshin 1 (SGOL1))

Abstract: SGOL1 Produits

Synonymes: anticorps sgo1, anticorps NY-BR-85, anticorps SGO, anticorps Sgo1, anticorps 3300001M08Rik, anticorps C81037, anticorps shugoshin 1, anticorps shugoshin centromeric cohesion 1, anticorps shugoshin 1 L homeolog, anticorps Sgo1, anticorps sgo1, anticorps sgo1.L, anticorps SGO1

Sujet: Shugoshin 1 (Sgo1), a meiosis-specific protein, protects centromeric Rec8 from degradation during first meiotic division (meiosis I) in fission yeast Schizosaccharomyces pombe. This protein appears at late prophase of meiosis I and is degraded at anaphase I. Heterochromatin protein Swi6 associates directly with Sgo1. A point mutation of Sgo1 (V242E), which abolishes the interaction with Swi6, impairs the centromeric localization and function of Sgo1. The forced centromeric localization of Sgo1 restores proper meiotic chromosome segregation in swi6 cells. Using this antibody in Western blotting, the band of 37 kD was observed in wild type S. pombe MP111 extract.

UniProt: Q9P7A0

Pathways: Maintenance of Protein Location