NSUN2
Reactivité: Humain
ELISA
Hôte: Lapin
Polyclonal
HRP
Indications d'application
NSUN2 antibody can be used for detection of NSUN2 by ELISA at 1:62500. NSUN2 antibody can be used for detection of NSUN2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.
Concentration
1 mg/mL
Buffer
Antibody is lyophilized in PBS buffer with 2 % sucrose.
Conseil sur la manipulation
As with any antibody avoid repeat freeze-thaw cycles.
Stock
4 °C/-20 °C
Stockage commentaire
For short periods of storage (days) store at 4 °C. For longer periods of storage, store NSUN2 antibody at -20 °C.
anticorps D13Wsu123e, anticorps Misu, anticorps MISU, anticorps MRT5, anticorps SAKI, anticorps TRM4, anticorps RGD1311954, anticorps NOL1/NOP2/Sun domain family member 2, anticorps NOP2/Sun RNA methyltransferase family member 2, anticorps NOP2/Sun RNA methyltransferase family, member 2, anticorps NOP2/Sun RNA methyltransferase family member 2 S homeolog, anticorps Nsun2, anticorps NSUN2, anticorps nsun2.S
Sujet
Maturation of cytoplasmic tRNAs includes splicing of introns, which are located 1 nucleotide 3-prime from the anticodon in all intron-containing tRNA genes. In tRNA-leu (CAA), the first position of the anticodon, C34, is converted to 5-methylcytosine, a modification necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. NSUN2 is a methyltransferase that catalyzes the intron-dependent formation of 5-methylcytosine at C34 of tRNA-leu (CAA).Maturation of cytoplasmic tRNAs includes splicing of introns, which are located 1 nucleotide 3-prime from the anticodon in all intron-containing tRNA genes. In tRNA-leu (CAA), the first position of the anticodon, C34, is converted to 5-methylcytosine, a modification necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. NSUN2 encodes a methyltransferase that catalyzes the intron-dependent formation of 5-methylcytosine at C34 of tRNA-leu (CAA) (Brzezicha et al., 2006 [PubMed 17071714]).