NARF anticorps
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- Antigène Voir toutes NARF Anticorps
- NARF (Nuclear Prelamin A Recognition Factor (NARF))
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Reactivité
- Humain
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp NARF est non-conjugé
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Application
- Western Blotting (WB), ELISA
- Purification
- Antibody is purified by peptide affinity chromatography method.
- Immunogène
- Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NARF.
- Top Product
- Discover our top product NARF Anticorps primaire
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- Indications d'application
- NARF antibody can be used for detection of NARF by ELISA at 1:312500. NARF antibody can be used for detection of NARF by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.
- Concentration
- 1 mg/mL
- Buffer
- Antibody is lyophilized in PBS buffer with 2 % sucrose.
- Conseil sur la manipulation
- As with any antibody avoid repeat freeze-thaw cycles.
- Stock
- 4 °C/-20 °C
- Stockage commentaire
- For short periods of storage (days) store at 4 °C. For longer periods of storage, store NARF antibody at -20 °C.
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- Antigène
- NARF (Nuclear Prelamin A Recognition Factor (NARF))
- Autre désignation
- NARF (NARF Produits)
- Synonymes
- anticorps NARF, anticorps IOP2, anticorps 4430402O11Rik, anticorps RGD1310894, anticorps wu:fa03c01, anticorps zgc:92186, anticorps nuclear prelamin A recognition factor, anticorps nuclear prelamin A recognition factor L homeolog, anticorps NARF, anticorps NAEGRDRAFT_78871, anticorps VDBG_04882, anticorps Tsp_07547, anticorps Tsp_07549, anticorps Narf, anticorps narf, anticorps narf.L
- Sujet
- Several proteins have been found to be prenylated and methylated at their carboxyl-terminal ends. Prenylation was initially believed to be important only for membrane attachment. However, another role for prenylation appears to be its importance in protein-protein interactions. The only nuclear proteins known to be prenylated in mammalian cells are prelamin A- and B-type lamins. Prelamin A is farnesylated and carboxymethylated on the cysteine residue of a carboxyl-terminal CaaX motif. This post-translationally modified cysteine residue is removed from prelamin A when it is endoproteolytically processed into mature lamin A. NARF binds to the prenylated prelamin A carboxyl-terminal tail domain. It may be a component of a prelamin A endoprotease complex. NARF is located in the nucleus, where it partially colocalizes with the nuclear lamina. It shares limited sequence similarity with iron-only bacterial hydrogenases.Several proteins have been found to be prenylated and methylated at their carboxyl-terminal ends. Prenylation was initially believed to be important only for membrane attachment. However, another role for prenylation appears to be its importance in protein-protein interactions. The only nuclear proteins known to be prenylated in mammalian cells are prelamin A- and B-type lamins. Prelamin A is farnesylated and carboxymethylated on the cysteine residue of a carboxyl-terminal CaaX motif. This post-translationally modified cysteine residue is removed from prelamin A when it is endoproteolytically processed into mature lamin A. The protein encoded by this gene binds to the prenylated prelamin A carboxyl-terminal tail domain. It may be a component of a prelamin A endoprotease complex. The encoded protein is located in the nucleus, where it partially colocalizes with the nuclear lamina. It shares limited sequence similarity with iron-only bacterial hydrogenases. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene, including one with a novel exon that is generated by RNA editing.
- Poids moléculaire
- 55 kDa
- ID gène
- 26502
- NCBI Accession
- NP_114174
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