NONO anticorps
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- Antigène Voir toutes NONO Anticorps
- NONO (Non-POU Domain Containing, Octamer-Binding (NONO))
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Reactivité
- Humain, Souris, Rat, Chien
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp NONO est non-conjugé
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Application
- Western Blotting (WB), ELISA
- Purification
- Antibody is purified by protein A chromatography method.
- Immunogène
- Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NONO.
- Top Product
- Discover our top product NONO Anticorps primaire
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- Indications d'application
- NONO antibody can be used for detection of NONO by ELISA at 1:312500. NONO antibody can be used for detection of NONO by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.
- Concentration
- 1 mg/mL
- Buffer
- Antibody is lyophilized in PBS buffer with 2 % sucrose.
- Conseil sur la manipulation
- As with any antibody avoid repeat freeze-thaw cycles.
- Stock
- 4 °C/-20 °C
- Stockage commentaire
- For short periods of storage (days) store at 4 °C. For longer periods of storage, store NONO antibody at -20 °C.
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- Antigène
- NONO (Non-POU Domain Containing, Octamer-Binding (NONO))
- Autre désignation
- NONO (NONO Produits)
- Synonymes
- anticorps NMT55, anticorps NRB54, anticorps P54, anticorps P54NRB, anticorps nmt55, anticorps nrb54, anticorps p54nrb, anticorps xp54nrb, anticorps AA407051, anticorps AV149256, anticorps nonA, anticorps non-POU domain containing octamer binding, anticorps non-POU domain containing, octamer-binding, anticorps non-POU domain containing, octamer binding L homeolog, anticorps non-POU-domain-containing, octamer binding protein, anticorps NONO, anticorps Nono, anticorps nono.L
- Sujet
- NONO is DNA- and RNA binding protein, involved in several nuclear processes. It binds the conventional octamer sequence in double stranded DNA. It also binds single-stranded DNA and RNA at a site independent of the duplex site. It is involved in pre-mRNA splicing and interacts with U5 snRNA. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs, be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1 and be involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V (D)J recombination and may stabilize paired DNA ends. NONO binds to an enhancer element in long terminal repeats of endogenous intracisternal A particles (IAPs) and activates transcription.
- Poids moléculaire
- 52 kDa
- ID gène
- 4841
- NCBI Accession
- NP_031389
- UniProt
- Q15233
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