MDFIC
Reactivité: Humain, Souris
ELISA, WB
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
MDFIC antibody can be used for detection of MDFIC by ELISA at 1:312500. MDFIC antibody can be used for detection of MDFIC by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.
Concentration
1 mg/mL
Buffer
Antibody is lyophilized in PBS buffer with 2 % sucrose.
Conseil sur la manipulation
As with any antibody avoid repeat freeze-thaw cycles.
Stock
4 °C/-20 °C
Stockage commentaire
For short periods of storage (days) store at 4 °C. For longer periods of storage, store MDFIC antibody at -20 °C.
Antigène
MDFIC
(MyoD Family Inhibitor Domain Containing (MDFIC))
anticorps HIC, anticorps Kdt1, anticorps Mdfid, anticorps MyoD family inhibitor domain containing, anticorps MyoD family inhibitor domain containing S homeolog, anticorps MDFIC, anticorps Mdfic, anticorps mdfic.S
Sujet
MDFIC is a member of a family of proteins characterized by a specific cysteine-rich C-terminal domain, which is involved in transcriptional regulation of viral genome expression. Alternative translation initiation from an upstream non-AUG (GUG), and an in-frame, downstream AUG codon, results in the production of two isoforms, p40 and p32, respectively, which have different subcellular localization, p32 is mainly found in the cytoplasm, whereas p40 is targeted to the nucleolus. Both isoforms have transcriptional regulatory activity that is attributable to the cysteine-rich C-terminal domain.This gene product is a member of a family of proteins characterized by a specific cysteine-rich C-terminal domain, which is involved in transcriptional regulation of viral genome expression. Alternative translation initiation from an upstream non-AUG (GUG), and an in-frame, downstream AUG codon, results in the production of two isoforms, p40 and p32, respectively, which have different subcellular localization, p32 is mainly found in the cytoplasm, whereas p40 is targeted to the nucleolus. Both isoforms have transcriptional regulatory activity that is attributable to the cysteine-rich C-terminal domain.