UBA3 anticorps
-
- Antigène Voir toutes UBA3 Anticorps
- UBA3 (Ubiquitin-Like Modifier Activating Enzyme 3 (UBA3))
-
Reactivité
- Humain, Souris, Rat
-
Hôte
- Lapin
-
Clonalité
- Polyclonal
-
Conjugué
- Cet anticorp UBA3 est non-conjugé
-
Application
- Western Blotting (WB), ELISA
- Purification
- Antibody is purified by peptide affinity chromatography method.
- Immunogène
- Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human UBA3.
- Top Product
- Discover our top product UBA3 Anticorps primaire
-
-
- Indications d'application
- UBA3 antibody can be used for detection of UBA3 by ELISA at 1:1562500. UBA3 antibody can be used for detection of UBA3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.
- Concentration
- 1 mg/mL
- Buffer
- Antibody is lyophilized in PBS buffer with 2 % sucrose.
- Conseil sur la manipulation
- As with any antibody avoid repeat freeze-thaw cycles.
- Stock
- 4 °C/-20 °C
- Stockage commentaire
- For short periods of storage (days) store at 4 °C. For longer periods of storage, store UBA3 antibody at -20 °C.
-
- Antigène
- UBA3 (Ubiquitin-Like Modifier Activating Enzyme 3 (UBA3))
- Autre désignation
- UBA3 (UBA3 Produits)
- Synonymes
- anticorps UBE1C, anticorps hUBA3, anticorps Ube1c, anticorps ube1c, anticorps wu:fb75e04, anticorps wu:fc37b11, anticorps zgc:55528, anticorps A830034N06Rik, anticorps AI256736, anticorps AI848246, anticorps AW546539, anticorps ubiquitin like modifier activating enzyme 3, anticorps ubiquitin-like modifier activating enzyme 3, anticorps ubiquitin like modifier activating enzyme 3 S homeolog, anticorps UBA3, anticorps Uba3, anticorps uba3, anticorps uba3.S
- Sujet
- UBA3 is a catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. UBA3 down-regulates steroid receptor activity. UBA3 is necessary for cell cycle progression.The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme associates with AppBp1, an amyloid beta precursor protein binding protein, to form a heterodimer, and then the enzyme complex activates NEDD8, a ubiquitin-like protein, which regulates cell division, signaling and embryogenesis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
- Poids moléculaire
- 52 kDa
- ID gène
- 9039
- NCBI Accession
- NP_003959
- UniProt
- Q8TBC4
-