Western Blotting (WB), Immunohistochemistry (IHC), ELISA
Purification
PPP4C antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogène
PPP4C antibody was raised against a 12 amino acid synthetic peptide near the C-Terminus of PPP4C.
ELISA: Antibody detection limit dilution 1:16000. Western Blot: Approximately 35 kDa band observed in HEK293, A431 and HeLa lysates (calculated MW of 35.1 kDa according to NP_002711.1). Recommended concentration: 0.1-0.3 μg/mL. Immunohistochemistry: In paraffin embedded human prostate shows nuclear staining of secretory cells in the gland. Recommended concentration, 2-4 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.