PCSK9 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogène
PCSK9 antibody was raised against a 12 amino acid synthetic peptide near the internal region of PCSK9 (aa 164-175).
PCSK9
Reactivité: Rat
WB, IHC, IP, ICC
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
ELISA: Antibody detection limit dilution 1:32000. Western Blot: In transfected HEK293 transiently expressing full-length human PCSK9 (myc and DYKDDDDK tagged), a doublet of approximately 80-100 kDa was observed. No bands were observed in mock-transfected HEK293 and the same bands were observed using anti-myc tag antibody. Recommended concentration, 0.1-0.5 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Minimize freezing and thawing.
Stock
-20 °C
Stockage commentaire
Aliquot and store at -20 °C.
Antigène
PCSK9
(Proprotein Convertase Subtilisin/kexin Type 9 (PCSK9))