Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
Specificité
This antibody may cross-react with AKR1C1
Purification
AKR1C4 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogène
AKR1C4 antibody was raised against a 13 amino acid synthetic peptide near the N-Terminus of AKR1C4.
AKR1C4
Reactivité: Humain
ELISA, IHC
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
ELISA: Antibody detection limit dilution 1:4000. Western Blot: Approximately 35 kDa band observed in human liver lysates (calculated MW of 37.1 kDa according to NP_001809.2). Recommended concentration: 0.1-0.3 μg/mL. Immunohistochemistry: In paraffin embedded human liver shows staining of distinct structures in the cytoplasm of hepatocytes. Recommended concentration, 2-4 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Minimize freezing and thawing.
Stock
-20 °C
Stockage commentaire
Aliquot and store at -20 °C.
Antigène
AKR1C4
(Aldo-keto Reductase Family 1 Member C4 (AKR1C4))