Cripto-1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogène
Cripto-1 antibody was raised against a 16 amino acid synthetic peptide near the internal region of Cripto-1.
ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 55 kDa band in human breast, placenta and testis lysates after 0.1 μg/mL antibody staining.6 kDa according to NP_115934.1. The 55 kDa band was successfully blocked by incubation with the immunizing peptide.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Minimize freezing and thawing.
Stock
-20 °C
Stockage commentaire
Aliquot and store at -20 °C.
Antigène
Cripto-1 (TDGF1.3)
(Teratocarcinoma-Derived Growth Factor 1, Member 3 (TDGF1.3))