HSD3B1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogène
HSD3B1 antibody was raised against a 12 amino acid synthetic peptide near the C-Terminus of HSD3B1.
HSD3B1
Reactivité: Humain
WB
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
ELISA: Antibody detection limit dilution 1:128000. Western Blot: Approximately 40 kDa band observed in human placenta lysates (calculated MW of 42.3 kDa according to NP_000853.1). In transfected HEK293 transiently expressing human HSD3B1 a band of approximately 45 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.01-0.1 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Minimize freezing and thawing.
Stock
-20 °C
Stockage commentaire
Aliquot and store at -20 °C.
Antigène
HSD3B1
(Hydroxy-delta-5-Steroid Dehydrogenase, 3 beta- and Steroid delta-Isomerase 1 (HSD3B1))