This antibody is expected to recognize all three reported isoforms (NP_000823.4, NP_067544.1, NP_015566.1).
Purification
NMDA Receptor antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogène
NMDA Receptor antibody was raised against a 12 amino acid synthetic peptide near the internal region of NMDA Receptor.
ELISA: Antibody detection limit dilution 1:1,000. Western Blot: Approximately 110 kDa band observed in rat brain lysates (calculated MW of 105 kDa according to NP_015566.1). Recommended concentration: 2-4 μg/mL. A minor band of unknown identity was also consistently observed at 23-24 kDa . This band was strong in mouse brain lysates and faint in human brain lysates. The band was successfully blocked by incubation with the immunizing peptide.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.