This antibody is expected to recognize all three human isoforms of this protein.
Purification
Nup50 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogène
Nup50 antibody was raised against a 12 amino acid synthetic peptide near the C-Terminus of Nup50.
NUP50
Reactivité: Humain, Souris
WB, ELISA
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 60 kDa band observed in Jurkat lysate (calculated MW 50.1 kDa according to NP_009103.2). Recommended for use at 0.2-1 μg/mL. Immunohistochemistry: In paraffin embedded human uterus shows textured nuclear staining of endometrial cells. Recommended concentration, 2.5-3.8 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.