S3-12 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogène
S3-12 antibody was raised against a 16 amino acid synthetic peptide near the internal region of S3-12.
ELISA: Antibody detection limit dilution 1:2,000. Western Blot: Approximately 45 kDa and 50 kDa in human adipose lysates after 0.1 μg/mL antibody staining.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). Immunohistochemistry: In paraffin embedded human pancreas shows membranous and cytoplasm staining in adipocytes. Recommended concentration, 3-6 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.