HSP90 anticorps (HRP)

N° du produit ABIN2481354

Détail du produit anti-HSP90 anticorps (HRP)

Antigène: HSP90 (Heat Shock Protein 90 (HSP90))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp HSP90 est conjugé à/à la HRP

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Antibody Array (AA)

Specificité: Detects 90 kDa. Detects HSP90 beta in all reactive species except in Chicken, where it detects both alpha and beta isoforms.

Réactivité croisée: Poulet, Chien, Poisson, Hamster, Humain, Souris, Lapin, Rat, Requin

Purification: Protein G Purified

Immunogène: Recombinant human HSP90beta

Clone: H9010

Isotype: IgG2a

Information d'application

Indications d'application:

• WB (1:2500)
• IHC (1:100)
• optimal dilutions for assays should be determined by the user.

Commentaires:

1 μg/ml of ABIN2481354 was sufficient for detection of HSP90beta in 20 μg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.2, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Conjugated antibodies should be stored at 4°C

Détails sur HSP90

Antigène: HSP90 (Heat Shock Protein 90 (HSP90))

Autre désignation: HSP90 (HSP90 Produits)

Synonymes: anticorps EL52, anticorps HSP86, anticorps HSP89A, anticorps HSP90A, anticorps HSP90N, anticorps HSPC1, anticorps HSPCA, anticorps HSPCAL1, anticorps HSPCAL4, anticorps HSPN, anticorps Hsp89, anticorps Hsp90, anticorps LAP2, anticorps git10, anticorps swo1, anticorps HSP90, anticorps htpG, anticorps SCBAC25F8.08, anticorps 23.m06066, anticorps 17.m07646, anticorps HSP90-1, anticorps 143198_at, anticorps 83, anticorps 83K HSP, anticorps DMHSP82, anticorps E(sev)3A, anticorps E(sina)2, anticorps HSP82, anticorps HSP83, anticorps ORF1, anticorps Su(Raf)3A, anticorps anon-EST:Liang-2.53, anticorps anon-WO0068693, anticorps anon-WO0140519.209, anticorps clone 2.53, anticorps en(lz)3C/4C, anticorps hsp84, anticorps l(3)j5C2, anticorps ms(3)08445, anticorps stc, anticorps DmelCG1242, anticorps CG1242, anticorps Hsp86, anticorps Hspca, anticorps 86kDa, anticorps 89kDa, anticorps AL024080, anticorps AL024147, anticorps Hsp86-1, anticorps hsp4, anticorps hsp86, anticorps hsp89, anticorps hsp90, anticorps hsp90a, anticorps hspc1, anticorps hspca, anticorps hspn, anticorps lap2, anticorps Hsp90alpha, anticorps heat shock protein 90 alpha family class A member 1, anticorps heat shock protein Hsp90, anticorps Hsp90 chaperone, anticorps Heat shock protein 90, anticorps heat shock protein 90, anticorps chaperone protein HtpG, anticorps molecular chaperone HtpG, anticorps Heat Shock Protein 90, anticorps uncharacterized LOC100384473, anticorps Heat shock protein 83, anticorps heat shock protein 90, alpha (cytosolic), class A member 1, anticorps heat shock protein 90 alpha family class B member 1, anticorps heat shock protein 90kDa alpha family class A member 1 L homeolog, anticorps heat shock protein HSP 90-alpha, anticorps HSP90AA1, anticorps hsp90, anticorps HSP90, anticorps daf-21, anticorps htpG, anticorps SCO7516, anticorps MAP_RS10510, anticorps TP04_0646, anticorps TP01_0934, anticorps APH_RS03525, anticorps GbCGDNIH1_0315, anticorps MAV_2118, anticorps HSP90C, anticorps BBOV_IV008400, anticorps BBOV_III007380, anticorps ACICU_00312, anticorps ECL_01244, anticorps YE105_C1172, anticorps pco153543(105), anticorps Hsp83, anticorps Hsp90aa1, anticorps HSP90AB1, anticorps hsp90aa1.1.L, anticorps LOC108698781

Sujet: HSP90 is an abundantly and ubiquitously expressed heat shock protein. It is understood to exist in two principal forms α and β, which share 85 % sequence amino acid homology. The two isoforms of HSP90, are expressed in the cytosolic compartment (1). Despite the similarities, HSP90α exists predominantly as a homodimer while HSP90β exists mainly as a monomer (2). From a functional perspective, HSP90 participates in the folding, assembly, maturation, and stabilization of specific proteins as an integral component of a chaperone complex (3-6). Furthermore, HSP90 is highly conserved between species, having 60 % and 78 % amino acid similarity between mammalian and the corresponding yeast and Drosophila proteins, respectively. HSP90 is a highly conserved and essential stress protein that is expressed in all eukaryotic cells. Despite it's label of being a heat-shock protein, HSP90 is one of the most highly expressed proteins in unstressed cells (1-2 % of cytosolic protein). It carries out a number of housekeeping functions - including controlling the activity, turnover, and trafficking of a variety of proteins. Most of the HSP90-regulated proteins that have been discovered to date are involved in cell signaling (7-8). The number of proteins now know to interact with HSP90 is about 100. Target proteins include the kinases v-Src, Wee1, and c-Raf, transcriptional regulators such as p53 and steroid receptors, and the polymerases of the hepatitis B virus and telomerase (5). When bound to ATP, HSP90 interacts with co-chaperones Cdc37, p23, and an assortment of immunophilin-like proteins, forming a complex that stabilizes and protects target proteins from proteasomal degradation. In most cases, HSP90-interacting proteins have been shown to co-precipitate with HSP90 when carrying out immunoadsorption studies, and to exist in cytosolic heterocomplexes with it. In a number of cases, variations in HSP90 expression or HSP90 mutation has been shown to degrade signaling function via the protein or to impair a specific function of the protein (such as steroid binding, kinase activity) in vivo. Ansamycin antibiotics, such as geldanamycin and radicicol, inhibit HSP90 function (9). For more information visit our HSP90 Scientific Resource Guide at http://www.HSP90.ca.

ID gène: 3326

NCBI Accession: NP_031381

UniProt: P08238

Pathways: M Phase, Regulation of Cell Size, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals