KDELR anticorps (AA 192-212) (APC)

N° du produit ABIN2481605

Détail du produit anti-KDELR anticorps (APC)

Antigène: KDELR (KDEL Receptor (KDELR))

Épitope: AA 192-212

Reactivité: Boeuf (Vache)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp KDELR est conjugé à/à la APC

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Specificité: Detects ~25 kDa.

Réactivité croisée: Poulet, Boeuf (Vache), Chien, Drosophila melanogaster, Hamster, Humain, Singe, Souris, Porc, Lapin, Rat, Mouton, Xenopus laevis

Purification: Protein G Purified

Immunogène: A 21 residue synthetic peptide (amino acids 192-212) based on the bovine KDEL receptor and the peptide coupled to KLH

Clone: KR-10

Isotype: IgG1

Information d'application

Indications d'application:

• WB (1:1000)
• ICC/IF (1:1000)
• optimal dilutions for assays should be determined by the user.

Commentaires:

1 μg/ml was sufficient for detection of KDEL receptor in 20 μg monkey Vero cell lysate by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:AP as the secondary.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.2, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Conjugated antibodies should be stored at 4°C

Détails sur KDELR

Antigène: KDELR (KDEL Receptor (KDELR))

Autre désignation: KDEL Receptor (KDELR Produits)

Synonymes: anticorps BEST:CK00230, anticorps CG5183, anticorps CK00230, anticorps Dmel\\CG5183, anticorps ERD2, anticorps Erd2, anticorps dmErd2, anticorps l(2)k00311, anticorps KDEL receptor, anticorps KdelR

Sujet: The endoplasmic reticulum is part of a protein sorting pathway, or in essence, the transportation system of the eukaryotic cell. The majority of endoplasmic reticulum resident proteins are retained in the endoplasmic reticulum through a retention motif. This motif is composed of four amino acids at the C-terminal end of the protein sequence. The most common retention sequence is KDEL (lys-asp-glu-leu). However, variation on KDEL does occur and other sequences can also give rise to endoplasmic reticulum retention (6). There are three KDEL receptors in mammalian cells, all have a very high degree of sequence identity, and all are located within the cis-Golgi and its intermediate compartments (4). In terms of function, KDEL receptors interact with GAP (GTPase-activating protein) of ARF1, which is involved in COPI dependent vesicle transport, and the KDEL receptor may also be responsible for the recruitment of this ARF1 to membranes which can then aid in the regulation of vesicle budding (3). It is also important to note that the KDEL receptor exhibits extensive sequence identity o yeast protein Erd2p, which is a receptor for the yeast ER retention signal (4, 5).

ID gène: 618184

NCBI Accession: NP_001069963

UniProt: P33946

Pathways: Maintenance of Protein Location