Calnexin anticorps (C-Term) (FITC)

N° du produit ABIN2481641

Détail du produit anti-Calnexin anticorps (FITC)

Antigène: Calnexin (CANX)

Épitope: C-Term

Reactivité: Chien

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Calnexin est conjugé à/à la FITC

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Specificité: Detects the C-terminal domain of Calnexin ~90 kDa. Weak detection in Chicken, Drosophila, and Xenopus tissues.

Réactivité croisée: Poulet, Boeuf (Vache), Chien, Drosophila melanogaster, Cobaye, Hamster, Humain, Singe, Souris, Porc, Caille, Lapin, Rat, Mouton, Xenopus laevis

Purification: Protein A Purified

Immunogène: Dog Calnexin C-terminal synthetic peptide conjugated to KLH. Identical to human, mouse and rat calnexin sequences over these residues.

Information d'application

Indications d'application:

• WB (1:2000)
• ICC/IF (1:100)
• IHC (1:100)
• optimal dilutions for assays should be determined by the user.

Commentaires:

A 1:2000 dilution of ABIN2481641 was sufficient for detection of Calnexin in 10 μg of HeLa cell lysate by ECL immunoblot analysis.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.2, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Conjugated antibodies should be stored at 4°C

Détails sur Calnexin

Antigène: Calnexin (CANX)

Autre désignation: Calnexin (CANX Produits)

Sujet: Calnexin, an abundant ~90 kDa integral protein of the endoplasmic reticulum, is also referred to as IP90, p88 and p90 (1). It consists of a large 50 kDa N-terminal calcium-binding luminal domain, a single transmembrane helix and a short acidic cytoplasmic tail (2, 3). Unlike its ER counterparts which have a KDEL sequence on their C-terminus to ensure ER retention (4), calnexin has positively charged cytosolic residues that do the same thing (3). Most ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into multi-subunit proteins. Calnexin together with calreticulin, plays a key role in glycoprotein folding and its control within the ER, by interacting with folding intermediates via their mono-glycosylated glycans (5, 6). Calnexin has also been shown to associate with the major histocompatibility complex class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin (7).

ID gène: 403908

NCBI Accession: NP_001003232

UniProt: P24643

Pathways: Signalisation MAPK, Thyroid Hormone Synthesis