HSPA4 anticorps (Atto 390)

N° du produit ABIN2481904

Détail du produit anti-HSPA4 anticorps (Atto 390)

Antigène: HSPA4 (Heat Shock 70kDa Protein 4 (HSPA4))

Reactivité: Hamster

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp HSPA4 est conjugé à/à la Atto 390

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificité: Detects ~110 kDa.

Réactivité croisée: Boeuf (Vache), Hamster, Humain, Singe, Souris, Rat, Saccharomyces cerevisiae, Requin, Mouton

Purification: Protein A Purified

Immunogène: Synthetic peptide derived from the sequence of hamster HSP110, sequence identical to human and mouse

Information d'application

Indications d'application:

• WB (1:1000)
• IHC (1:100)
• optimal dilutions for assays should be determined by the user.

Commentaires:

1 μg/ml of ABIN2481904 was sufficient for detection of HSP110 in 10 μg of human cell line mixed lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.2, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Conjugated antibodies should be stored at 4°C

Détails sur HSPA4

Antigène: HSPA4 (Heat Shock 70kDa Protein 4 (HSPA4))

Autre désignation: HSP110 (HSPA4 Produits)

Sujet: HSP110 belongs to a family of large stress proteins known as the HSP110/SSE Family. The proteins in this family are the most distantly known relatives of the well studied HSP70 family. They share 30-33 % amino acid identity, mostly in the conserved ATP-binding domain (1). HSP110 cooperates with HSP70 in protein folding in the eukaryotic cytosol (2). In mammals, HSP110 is constitutively expressed, but exhibits particularly high levels in the brain. Both HSP70 and HSP110 are elevated after cerebral ischemia. Recent studies demonstrate that the protective effects of HSP110 deficiency in cerebral ischemia may partly be mediated by an increase in the chaperone activity of HSP70 (3). Studies also suggest that HSP110 can be used in heat shock protein-based cancer immunotherapy (4).

ID gène: 10808

NCBI Accession: NP_006635

UniProt: Q92599