Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

Notch1 anticorps (AA 20-216) (PerCP)

NOTCH1 Reactivité: Souris WB, IF, ICC Hôte: Souris Monoclonal S253-32 PerCP
N° du produit ABIN2483203
  • Antigène Voir toutes Notch1 (NOTCH1) Anticorps
    Notch1 (NOTCH1) (Notch 1 (NOTCH1))
    Épitope
    • 16
    • 16
    • 12
    • 10
    • 7
    • 5
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 20-216
    Reactivité
    • 124
    • 92
    • 49
    • 10
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Souris
    Hôte
    • 99
    • 39
    • 6
    • 4
    • 3
    • 2
    • 1
    Souris
    Clonalité
    • 95
    • 55
    • 2
    Monoclonal
    Conjugué
    • 70
    • 11
    • 9
    • 9
    • 8
    • 6
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Cet anticorp Notch1 est conjugé à/à la PerCP
    Application
    • 95
    • 51
    • 48
    • 33
    • 27
    • 26
    • 18
    • 17
    • 16
    • 10
    • 10
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
    Specificité
    Detects >270 kDa. Does not cross-react with Notch 2 or Notch3.
     Réactivité croisée
    Humain, Souris, Rat
    Purification
    Protein G Purified
    Immunogène
    Fusion protein amino acids 20-216 (extracellular N-terminus, EGF-like domains 1-5) of mouse Notch1
    Clone
    S253-32
    Isotype
    IgG1
    Top Product
    Discover our top product NOTCH1 Anticorps primaire
  • Indications d'application
    • WB (1:1000)
    • optimal dilutions for assays should be determined by the user.
    Commentaires

    1 μg/ml of ABIN2483203 was sufficient for detection of Notch1 in 20 μg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C
    Stockage commentaire
    Conjugated antibodies should be stored at 4°C
  • Antigène
    Notch1 (NOTCH1) (Notch 1 (NOTCH1))
    Autre désignation
    Notch1 (NOTCH1 Produits)
    Synonymes
    anticorps notch1, anticorps NOTCH1, anticorps hn1, anticorps tan1, anticorps notch, anticorps xotch, anticorps xnotch, anticorps notch-1, anticorps xnotch1, anticorps TAN1, anticorps hN1, anticorps 9930111A19Rik, anticorps Mis6, anticorps N1, anticorps Tan1, anticorps lin-12, anticorps NOTCH, anticorps Xotch, anticorps notch1-a, anticorps notch 1, anticorps fibropellin-1, anticorps Notch homolog 1, translocation-associated (Drosophila), anticorps Notch homolog 1, translocation-associated, anticorps notch 1 S homeolog, anticorps notch1, anticorps LOC100631526, anticorps NOTCH1, anticorps Notch1, anticorps notch1.S
    Sujet
    Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane.
    ID gène
    18128
    NCBI Accession
    NP_032740
    UniProt
    Q01705
    Pathways
    Signalisation Notch, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development
Vous êtes ici:
Support technique