SHANK1 anticorps (AA 469-691) (HRP)

N° du produit ABIN2483711

Détail du produit anti-SHANK1 anticorps (HRP)

Antigène: SHANK1 (SH3 and Multiple Ankyrin Repeat Domains 1 (SHANK1))

Épitope: AA 469-691

Reactivité: Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp SHANK1 est conjugé à/à la HRP

Application: Western Blotting (WB), Immunohistochemistry (IHC), Antibody Array (AA)

Specificité: Detects ~190-220 kDa (alternative splice variants). No cross-reactivity against Shank2 or Shank3.

Réactivité croisée: Humain, Souris, Rat

Purification: Protein G Purified

Immunogène: Fusion protein amino acids 469-691 (SH3/PDZ domains) of rat Shank1

Clone: N22-21 (Formerly S22-21)

Isotype: IgG1

Information d'application

Indications d'application:

• WB (1:1000)
• IHC (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Commentaires:

1 μg/ml of ABIN2483711 was sufficient for detection of Shank1 in 10 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Conjugated antibodies should be stored at 4°C

Détails sur SHANK1

Antigène: SHANK1 (SH3 and Multiple Ankyrin Repeat Domains 1 (SHANK1))

Autre désignation: SHANK1 (SHANK1 Produits)

Synonymes: anticorps SPANK-1, anticorps SSTRIP, anticorps synamon, anticorps Shank1a, anticorps Spank1, anticorps Sstrip, anticorps SH3 and multiple ankyrin repeat domains 1, anticorps SHANK1, anticorps Shank1

Sujet: Shank proteins make up a family of scaffold proteins identified through their interaction with a variety of membrane and cytoplasmic proteins (1). Shank proteins at postsynaptic sites of excitatory synapses play roles in signal transmission into the postsynaptic neuron. Studies suggest that Shank2 is expressed in the neurons of the developing retina, and could play a role in the neuronal differentiation of the developing retina (2). Other recent studies suggest that the disruption of glutamate receptors at the Shank postsynaptic platform could contribute to the destruction of the postsynaptic density, which underlies the synaptic dysfunction and loss in Alzheimer's disease (3).

ID gène: 78957

NCBI Accession: NP_113939

UniProt: Q9WV48

Pathways: Synaptic Membrane, Maintenance of Protein Location