PDIA3 anticorps (Biotin)
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- Antigène Voir toutes PDIA3 Anticorps
- PDIA3 (Protein Disulfide Isomerase Family A, Member 3 (PDIA3))
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp PDIA3 est conjugé à/à la Biotin
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- Specificité
- Detects ~57 kDa.
- Réactivité croisée
- Boeuf (Vache), Chien, Cobaye, Hamster, Humain, Singe, Souris, Porc, Lapin, Rat
- Purification
- Protein G Purified
- Immunogène
- Human recombinant ERp57 (Grp58)
- Clone
- Map-ERp57
- Isotype
- IgG1
- Top Product
- Discover our top product PDIA3 Anticorps primaire
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- Indications d'application
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- WB (1:2000)
- IHC (1:100)
- ICC/IF (1:100)
- optimal dilutions for assays should be determined by the user.
- Commentaires
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0.5 μg/ml of ABIN2484406 was sufficient for detection of ERp57 in 10 μg of heat shock Heal Lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C
- Stockage commentaire
- Conjugated antibodies should be stored at 4°C
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- Antigène
- PDIA3 (Protein Disulfide Isomerase Family A, Member 3 (PDIA3))
- Autre désignation
- ERp57 (PDIA3 Produits)
- Sujet
- ERp57, also known as Glucose Regulated Protein 58 (Grp58), Hormone-Induced Protein-70 (HIP-70) and microsomal Carnitine Palmitoyltransferase, is a member of the protein disulfide isomerase family, containing two canonical CXHC tetrapeptide active site motifs (1-5). It has quite a few diverse roles. It functions as an accessory oxidoreductase involved in disulfide bond formation. In the ER, ERp57 interacts with membrane bound calnexin and soluble calreticulin (lectin chaperones) via their praline rich P-domain arms. Lectin chaperones bind nascent non-native glycoproteins, and position ERp57 to act upon the immature or misfolded glycoproteins that possess mono-glycosylated side chains. ERp57 deletion impairs posttranslational phases of influenza hema-glutinin folding, and causes accelerated release of MHC-I molecules, resulting in the coupling of sub-optimal peptides and reduced expression and stability on the cell surface (6). ERp57 also contains two thioredoxin active-site sequences, CGHC and an estrogen-binding domain. ERp57 is induced by both estrogen and leuteinizing-hormone-releasing hormone in the hippocampus (7).
- ID gène
- 2923
- NCBI Accession
- NP_005304
- UniProt
- P30101
- Pathways
- Maintenance of Protein Location, Protein targeting to Nucleus, Cell RedoxHomeostasis
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