PDIA3 anticorps (Biotin)

N° du produit ABIN2484406

Détail du produit anti-PDIA3 anticorps (Biotin)

Antigène: PDIA3 (Protein Disulfide Isomerase Family A, Member 3 (PDIA3))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PDIA3 est conjugé à/à la Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Specificité: Detects ~57 kDa.

Réactivité croisée: Boeuf (Vache), Chien, Cobaye, Hamster, Humain, Singe, Souris, Porc, Lapin, Rat

Purification: Protein G Purified

Immunogène: Human recombinant ERp57 (Grp58)

Clone: Map-ERp57

Isotype: IgG1

Information d'application

Indications d'application:

• WB (1:2000)
• IHC (1:100)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Commentaires:

0.5 μg/ml of ABIN2484406 was sufficient for detection of ERp57 in 10 μg of heat shock Heal Lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Conjugated antibodies should be stored at 4°C

Détails sur PDIA3

Antigène: PDIA3 (Protein Disulfide Isomerase Family A, Member 3 (PDIA3))

Autre désignation: ERp57 (PDIA3 Produits)

Sujet: ERp57, also known as Glucose Regulated Protein 58 (Grp58), Hormone-Induced Protein-70 (HIP-70) and microsomal Carnitine Palmitoyltransferase, is a member of the protein disulfide isomerase family, containing two canonical CXHC tetrapeptide active site motifs (1-5). It has quite a few diverse roles. It functions as an accessory oxidoreductase involved in disulfide bond formation. In the ER, ERp57 interacts with membrane bound calnexin and soluble calreticulin (lectin chaperones) via their praline rich P-domain arms. Lectin chaperones bind nascent non-native glycoproteins, and position ERp57 to act upon the immature or misfolded glycoproteins that possess mono-glycosylated side chains. ERp57 deletion impairs posttranslational phases of influenza hema-glutinin folding, and causes accelerated release of MHC-I molecules, resulting in the coupling of sub-optimal peptides and reduced expression and stability on the cell surface (6). ERp57 also contains two thioredoxin active-site sequences, CGHC and an estrogen-binding domain. ERp57 is induced by both estrogen and leuteinizing-hormone-releasing hormone in the hippocampus (7).

ID gène: 2923

NCBI Accession: NP_005304

UniProt: P30101

Pathways: Maintenance of Protein Location, Protein targeting to Nucleus, Cell RedoxHomeostasis