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TCP1 alpha/CCTA anticorps (C-Term) (FITC)

TCP1 Reactivité: Souris WB, IF, IP, FACS, ICC Hôte: Rat Monoclonal 91a FITC
N° du produit ABIN2485921
  • Antigène Voir toutes TCP1 alpha/CCTA (TCP1) Anticorps
    TCP1 alpha/CCTA (TCP1) (T-Complex 1 (TCP1))
    Épitope
    • 40
    • 18
    • 18
    • 15
    • 15
    • 15
    • 15
    • 9
    • 5
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivité
    • 173
    • 121
    • 79
    • 22
    • 21
    • 20
    • 19
    • 18
    • 17
    • 15
    • 13
    • 11
    • 10
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    Souris
    Hôte
    • 153
    • 27
    • 19
    • 1
    • 1
    Rat
    Clonalité
    • 147
    • 54
    Monoclonal
    Conjugué
    • 68
    • 14
    • 11
    • 10
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    Cet anticorp TCP1 alpha/CCTA est conjugé à/à la FITC
    Application
    • 129
    • 53
    • 52
    • 45
    • 44
    • 41
    • 40
    • 27
    • 24
    • 23
    • 13
    • 11
    • 3
    • 2
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunocytochemistry (ICC)
    Specificité
    Detects ~60 & ~92 kDa. Cross reactivity with human HSP60 in WB. Reacts weakly with S. cerevisiae, consistent with the epitope being AKLRS (instead of AKLRA). In C. elegans, it reacts with TCP1 alpha and another CCT subunit. In plants, it recog TCP1 of P. sativum, and the sequence of A. thalania TCP1 over the region of the epitope AKLRA. It has also been shown that it reacts with a subunit of a specialized chaperonin which folds phytochrome.
     Réactivité croisée
    C. elegans, Boeuf (Vache), Chien, Drosophila melanogaster, Cobaye, Hamster, Humain, Singe, Souris, Porc, Lapin, Rat, Saccharomyces cerevisiae
    Purification
    Protein G Purified
    Immunogène
    Recombinant Mouse TCP1 alpha protein fragment (carboxy terminal region).
    Clone
    91a
    Isotype
    IgG2a
    Top Product
    Discover our top product TCP1 Anticorps primaire
  • Indications d'application
    • WB (1:1000)
    • ICC/IF (1:100)
    • optimal dilutions for assays should be determined by the user.
    Commentaires

    1 μg/ml of ABIN2485921 was sufficient for detection of TCP1 alpha in 20 μg of 3T3 cell lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.4, 50 % glycerol, 0.1 % sodium azide, Storage buffer may change when conjugated
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C
    Stockage commentaire
    Conjugated antibodies should be stored at 4°C
  • Antigène
    TCP1 alpha/CCTA (TCP1) (T-Complex 1 (TCP1))
    Autre désignation
    TCP1 alpha (TCP1 Produits)
    Synonymes
    anticorps CCT-alpha, anticorps CCT1, anticorps CCTa, anticorps D6S230E, anticorps TCP-1-alpha, anticorps AI528772, anticorps CCT, anticorps Cct1, anticorps Ccta, anticorps TRic, anticorps Tcp-1, anticorps Tp63, anticorps c-cpn, anticorps p63, anticorps TRiC, anticorps CCTalpha, anticorps BEST:GH05123, anticorps CG5374, anticorps Dmel\\CG5374, anticorps T-cpl, anticorps TCP-1alpha, anticorps TCPA_DROME, anticorps Tcp1, anticorps Tcp1-alpha, anticorps gh05123, anticorps cct-alpha, anticorps ccta, anticorps tcp1, anticorps tcp1-a, anticorps tcp1a, anticorps tcp1alpha, anticorps CHUNP6875, anticorps fa13h08, anticorps wu:fa13h08, anticorps wu:fc95g06, anticorps t-complex 1, anticorps T-complex protein 1 subunit alpha, anticorps t-complex protein 1, anticorps Tcp1-like, anticorps t-complex 1 S homeolog, anticorps TCP1, anticorps cct-1, anticorps Tcp1, anticorps T-cp1, anticorps tcp1.S, anticorps tcp1
    Sujet
    T-complex polypeptide-1 (TCP1) is a ~60 kDa protein constitutively expressed in almost all eukaryotic cells, and is up-regulated during spermatogenesis. It is found in the cytosol as a subunit of a hetero-oligomeric chaperone that is known to be involved in the folding of actin and tubulin. The family of proteins termed chaperonins act to recognize and stabilize polypeptide intermediates during folding, assembly and disassembly, and share many characteristics with Heat Shock Protein 70 (HSP 70) including high abundance, induction by environmental stress, and ATPase activity. The chaperonin family includes the mitochondrial HSP60, Escherichia coli GroEL, the plastid Rubisco-subunit binding protein, and the archaebacterial protein TF55. The TCP1 sequence shows nearly 40 % identity to TF55, but only minimal similarity to HSP60 and GroEL.
    ID gène
    21454
    NCBI Accession
    NP_038714
    UniProt
    P11983
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