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RAB5 anticorps (PerCP)

RAB5A Reactivité: Humain WB, IHC, IF, ICC Hôte: Lapin Polyclonal PerCP
N° du produit ABIN2486179
  • Antigène Voir toutes RAB5 (RAB5A) Anticorps
    RAB5 (RAB5A) (RAB5A, Member RAS Oncogene Family (RAB5A))
    Reactivité
    • 95
    • 64
    • 45
    • 11
    • 11
    • 10
    • 9
    • 9
    • 8
    • 8
    • 7
    • 6
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    Humain
    Hôte
    • 97
    • 10
    • 3
    • 2
    Lapin
    Clonalité
    • 94
    • 18
    Polyclonal
    Conjugué
    • 50
    • 8
    • 7
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp RAB5 est conjugé à/à la PerCP
    Application
    • 82
    • 39
    • 35
    • 33
    • 24
    • 18
    • 14
    • 13
    • 13
    • 7
    • 4
    • 4
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
    Specificité
    Detects ~26 kDa.
     Réactivité croisée
    Boeuf (Vache), Humain, Singe, Souris, Rat
    Purification
    Protein A Purified
    Immunogène
    Human Rab5 synthetic peptide conjugated to KLH, identical to dog Rab5 sequence over the residues
    Top Product
    Discover our top product RAB5A Anticorps primaire
  • Indications d'application
    • WB (1:1000)
    • IHC (1:100)
    • ICC/IF (1:80)
    • optimal dilutions for assays should be determined by the user.
    Commentaires

    1 μl/ml of ABIN2486179 was sufficient for detection of Rab5 in 15 μg of HeLa lysate by ECL immunoblot analysis using Donkey anti-rabbit IgG:HRP as the secondary antibody.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C
    Stockage commentaire
    Conjugated antibodies should be stored at 4°C
  • Antigène
    RAB5 (RAB5A) (RAB5A, Member RAS Oncogene Family (RAB5A))
    Autre désignation
    Rab5 (RAB5A Produits)
    Synonymes
    anticorps RAB5, anticorps 2410015H04Rik, anticorps AI663973, anticorps AU021172, anticorps nnyRab5a, anticorps DDBDRAFT_0168594, anticorps DDBDRAFT_0229401, anticorps DDB_0168594, anticorps DDB_0229401, anticorps rab5, anticorps RAB5A, anticorps Rab5a, anticorps rab5al, anticorps zgc:56644, anticorps fj38g08, anticorps hm:zehn1144, anticorps rab5a, anticorps wu:fj38g08, anticorps RAB5A, member RAS oncogene family, anticorps rab5A protein, anticorps Rab5a, GTPase, anticorps predicted protein, anticorps Rab GTPase, anticorps RAB5A, member RAS oncogene family L homeolog, anticorps RAB5A, member RAS oncogene family, b, anticorps RAB5A, member RAS oncogene family, a, anticorps RAB5A, anticorps Rab5a, anticorps rab5A, anticorps rab5a, anticorps rab5a.L, anticorps rab5ab, anticorps rab5aa
    Sujet
    Rab5 is a 24 kDa member of the Rab family of small guanosine triphosphatases (GTPases), Ras superfamily. Rab GTPases are central regulators of membrane trafficking in the eukaryotic cell. Their regulatory capacity depends on their ability to cycle between the GDP -bound inactive and GTP-bound active states. This conversion is regulated by GDP/GTP exchange factors (GEPs), GDP dissociation inhibitors (GDIs) and GTPase-activating proteins (GAPs) (1, 2). Activation of a Rab protein is coupled to its association with intracellular membranes, allowing it to recruit downstream effector proteins to the cytoplasmic surface of a subcellular compartment (3). Through these proteins, Rab GTPases regulate vesicle formation, actin- and tubulin-dependent vesicle movement, and membrane fusion(1). Rab proteins contain conserved regions involved in guanine-nucleotide binding, and hyper variable COHO-terminal domains with a cysteine motif implicated in subcellular targeting. Post-translational modification of the cysteine motif with one or two geranyl groups is essential for the membrane association and correct intracellular localization of Rab proteins(3). Each Rab shows a characteristic subcellular distribution (4). In particular, Rab5 is ubiquitously expressed in human tissues. It localizes mainly to early endosomes, but is also present on the plasma membrane. It regulates the fusion between endocytic vesicles and early endosomes, as well as the homotypic fusion between early endosomes (5). Among the proteins recruited by the GTP-bound active Rab5 are Rabaptin-5 and EEA1 (6). Anti-Rab5 may be used as an early endosome marker.
    ID gène
    5868
    NCBI Accession
    NP_004153
    UniProt
    P20339
    Pathways
    Smooth Muscle Cell Migration, Regulation of long-term Neuronal Synaptic Plasticity
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