Nitrotyrosine anticorps (Atto 488)

N° du produit ABIN2486183

Détail du produit anti-Nitrotyrosine anticorps (Atto 488)

Antigène: Nitrotyrosine

Reactivité: Veuillez nous consulter SVP

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Nitrotyrosine est conjugé à/à la Atto 488

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Antibody Array (AA)

Specificité: Recognizes 3-nitrotyrosine moieties. No detectable cross-reactivity with non-nitrated tyrosine. Not species specific.

Purification: Protein G Purified

Immunogène: 3-(4-hydroxy-3-nitrophenylacetamido) propionic acid-bovine serum albumin

Clone: 39B6

Isotype: IgG2a

Information d'application

Indications d'application:

• WB (1:1400)
• IHC (1:100)
• optimal dilutions for assays should be determined by the user.

Commentaires:

0.7 μg/ml of ABIN2486183 was sufficient for detection of 5 μg SIN-1 treated BSA by Western Blot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Conjugated antibodies should be stored at 4°C

Détails sur Nitrotyrosine

Antigène: Nitrotyrosine

Abstract: Nitrotyrosine Produits

Classe de substances: Chemical

Sujet: Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxide signaling (2). While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosino-philperoxidase (3), myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Nitrotyrosine may also serve as a biomarker for the effects of reactive nitrogen oxides, based on tyrosine residues becoming nitrated in proteins at sites of inflammation induced tissue injury (1). The presence of nitro tyrosine-containing proteins therefore has shown high correlation to disease states such as atherosclerosis, Alzheimer's disease, Parkinson's disease and amyotrophic lateral sclerosis (4).