GRP78 anticorps (PerCP)

N° du produit ABIN2486762

Détail du produit anti-GRP78 anticorps (PerCP)

Antigène: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GRP78 est conjugé à/à la PerCP

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Specificité: Detects ~78 kDa.

Réactivité croisée: Boeuf (Vache), Chien, Fusarium, Hamster, Humain, Singe, Souris, Lapin, Rat, Xenopus laevis

Purification: Peptide Affinity Purified

Immunogène: Rat GRP78 (Bip) synthetic peptide conjugated to KLH

Information d'application

Indications d'application:

• WB (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Commentaires:

A 1:1000 dilution of ABIN2486762 was sufficient for detection of Grp78 in 10 μg of rat tissue lysate by ECL immunoblot analysis.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Conjugated antibodies should be stored at 4°C

Détails sur GRP78

Antigène: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Autre désignation: GRP78 (HSPA5 Produits)

Sujet: GRP78 is a ubiquitously expressed, 78- kDa glucose regulated protein, and is commonly referred to as an immunoglobin chain binding protein (BiP). The BiP proteins are categorized as stress response proteins because they play an important role in the proper folding and assembly of nascent protein and in the scavenging of misfolded proteins in the endoplasmic reticulum lumen. Translation of BiP is directed by an internal ribosomal entry site (IRES) in the 5' non-translated region of the BiP mRNA. BiP IRES activity increases when cells are heat stressed (1). GRP78 is also critical for maintenance of cell homeostasis and the prevention of apoptosis (2). Luo et al. have provided findings that suggest GRP78 is essential for embryonic cell growth and pluripotent cell survival (3). In terms of diseases, GRP78 has been shown to be a reliable biomarker of hypoglycemia, to serve a neuroprotective function in neurons exposed to glutamate and oxidative stress (4), and its protein levels are reduced in the brains of Alzheimer's patients (5). Also, the induction of the GRP78 protein that results in severe glucose and oxygen deprivation could possible lead to drug resistance to anti-tumor drugs (6, 7).

ID gène: 25617

NCBI Accession: NP_037215

UniProt: P06761

Pathways: Thyroid Hormone Synthesis, ER-Nucleus Signaling