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P4HB anticorps (AA 409-509) (PE)

P4HB Reactivité: Rat WB, IHC, IP, IF, ICC Hôte: Lapin Polyclonal PE
N° du produit ABIN2486780
  • Antigène Voir toutes P4HB Anticorps
    P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))
    Épitope
    • 20
    • 20
    • 15
    • 13
    • 11
    • 5
    • 4
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 409-509
    Reactivité
    • 109
    • 77
    • 66
    • 23
    • 22
    • 21
    • 20
    • 18
    • 18
    • 16
    • 10
    • 10
    • 8
    • 7
    • 6
    • 4
    • 4
    • 1
    • 1
    • 1
    Rat
    Hôte
    • 116
    • 29
    • 2
    Lapin
    Clonalité
    • 110
    • 37
    Polyclonal
    Conjugué
    • 64
    • 16
    • 15
    • 9
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp P4HB est conjugé à/à la PE
    Application
    • 113
    • 68
    • 45
    • 43
    • 43
    • 38
    • 18
    • 16
    • 15
    • 13
    • 13
    • 5
    • 5
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)
    Specificité
    Detects ~58 kDa.
     Réactivité croisée
    Boeuf (Vache), Chien, Cobaye, Hamster, Humain, Mollusca, Souris, Porc, Rat, Mouton, Xenopus laevis
    Purification
    Peptide Affinity Purified
    Immunogène
    Rat PDI synthetic peptide (409-509 aa C-terminal) conjugated to KLH
    Top Product
    Discover our top product P4HB Anticorps primaire
  • Indications d'application
    • WB (1:1000)
    • ICC/IF (1:100)
    • optimal dilutions for assays should be determined by the user.
    Commentaires

    A 1:1000 dilution of ABIN2486780 was sufficient for detection of PDI in 20 μg of HeLa cell lysate by ECL immunoblot analysis.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C
    Stockage commentaire
    Conjugated antibodies should be stored at 4°C
  • Antigène
    P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))
    Autre désignation
    PDI (P4HB Produits)
    Synonymes
    anticorps DSI, anticorps ERBA2L, anticorps GIT, anticorps P4Hbeta, anticorps PDI, anticorps PDIA1, anticorps PHDB, anticorps PO4DB, anticorps PO4HB, anticorps PROHB, anticorps PDA2, anticorps PDIP, anticorps PDIR, anticorps ERp59, anticorps Pdia1, anticorps Thbp, anticorps p55, anticorps XPDIp, anticorps pdi, anticorps 1810041F13Rik, anticorps AI661267, anticorps Pdip, anticorps Pdipl, anticorps p58, anticorps prolyl 4-hydroxylase subunit beta, anticorps protein disulfide isomerase family A member 2, anticorps prolyl 4-hydroxylase, beta polypeptide, anticorps protein disulfide isomerase family A member 2 L homeolog, anticorps protein disulfide isomerase associated 2, anticorps prolyl 4-hydroxylase subunit beta L homeolog, anticorps protein disulfide-isomerase, anticorps P4HB, anticorps PDIA2, anticorps P4hb, anticorps pdia2.L, anticorps Pdia2, anticorps p4hb.L, anticorps LOC8281065
    Sujet
    The three dimensional structure of many extracellular proteins is stabilized by the formation of disulphide bonds. Studies suggest that a microsomal enzyme known as Protein Disulphide Isomerase (PDI) is involved in disulphide-bond formation via its oxidase activity and isomerization via its isomerase activity, as well as the reduction of disulphide bonds in proteins (1). Studies suggest BiP and PDI work together sequentially to increase oxidation of these proteins (2, 3). PDI has also been found to function as a chaperone to prevent the aggregation of unfolded substrates, and serves as a subunit of prolyl 4-hydroxylase and microsomal triglyceride transferase (4, 5). PDI is an abundant 55 kDa protein located primarily in the ER, however studies have also proved its presence in the cytosol (1). PDI has the ability to reside in the ER permanently due to the highly conserved KDEL sequence at its carboxy-terminus (6). It uses carboxy-terminal KDEL as a retention signal, and this appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor (7).
    ID gène
    287164
    NCBI Accession
    NP_001099245
    UniProt
    P04785
    Pathways
    Maintenance of Protein Location, Cell RedoxHomeostasis, Lipid Metabolism
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