The antibody detects endogenous levels of RSK1/2/3/4 only when phosphorylated at serine 221/227/218/232.
Purification
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Immunogène
RSK1/2/3/4 (phospho Ser221/227/S218/232) antibody was raised against a peptide sequence around phosphorylation site of Serine 221/227/218/232 (A-Y-S (p)-F-C) derived from Human RSK1/2/3/4.
Reactivité: Humain, Souris
Hôte: Lapin
Polyclonal
Alexa Fluor 647
Indications d'application
Western Blot: 1:500~1:1000
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
-20 °C
Stockage commentaire
Store antibody at -20°C for up to one year.
Antigène
RSK1/2/3/4
Sujet
This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway.