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AIF anticorps (AA 183-195)

Verified AIFM1 Reactivité: Humain, Souris WB, IHC, ELISA, IF Hôte: Chèvre Polyclonal unconjugated
N° du produit ABIN2613375
  • Antigène Voir toutes AIF (AIFM1) Anticorps
    AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))
    Épitope
    • 15
    • 10
    • 8
    • 8
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 183-195
    Reactivité
    • 103
    • 56
    • 55
    • 22
    • 11
    • 11
    • 7
    • 7
    • 6
    • 5
    • 4
    • 4
    • 3
    • 2
    • 1
    • 1
    Humain, Souris
    Hôte
    • 92
    • 9
    • 2
    • 1
    Chèvre
    Clonalité
    • 88
    • 15
    Polyclonal
    Conjugué
    • 62
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp AIF est non-conjugé
    Application
    • 79
    • 33
    • 29
    • 27
    • 22
    • 14
    • 13
    • 13
    • 12
    • 7
    • 5
    • 4
    • 2
    • 2
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)
    Fonction
    AIFM1 (aa183-195)
    Séquence
    DDPNVTKTLR FKQ
    Specificité
    This antibody is expected to recognize isoform 1 (NP_004199.1), isoform 2 (NP_665811.1) and isoform 5 (NP_001124319).
     Réactivité croisée
    Boeuf (Vache), Humain
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Classe de qualité
    Verified
    Immunogène
    Peptide with sequence C-DDPNVTKTLRFKQ, from the internal region of the protein sequence
    Isotype
    IgG
    Top Product
    Discover our top product AIFM1 Anticorps primaire
  • Indications d'application

    Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 5-7 μg/mL.

    Western Blot: Approx 70 kDa band observed in lysates of cell line Jurkat (calculated MW of 66.9 kDa according to NP_004199.1). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 hour at room temperature.

    Peptide ELISA: antibody detection limit dilution 1:128000.

    Commentaires

    Immunofluorescence: Strong expression of the protein seen in the Mitochondria of HeLa and NIH3T3 cells. Recommended concentration: 5-10μg/ml.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Minimize freezing and thawing.
    Stock
    -20 °C
    Stockage commentaire
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Antigène
    AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))
    Autre désignation
    AIFM1 (AIFM1 Produits)
    Sujet
    AIFM1, apoptosis-inducing factor, mitochondrion-associated, 1, AIF, CMTX4, COWCK, COXPD6, PDCD8, apoptosis-inducing factor 1, mitochondrial, programmed cell death 8 (apoptosis-inducing factor), striatal apoptosis-inducing factor
    ID gène
    9131
    NCBI Accession
    NP_004199, NP_665811, NP_001124319
    Pathways
    Apoptose, Positive Regulation of Endopeptidase Activity, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, L'effet Warburg
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