Vimentin anticorps (C-Term)
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- Antigène Voir toutes Vimentin (VIM) Anticorps
- Vimentin (VIM)
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Épitope
- C-Term
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Reactivité
- Humain, Souris, Rat
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Hôte
- Chèvre
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp Vimentin est non-conjugé
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
- Fonction
- Vimentin
- Séquence
- QVINETSQHH DDLE
- Réactivité croisée
- Boeuf (Vache), Chien, Humain, Souris, Porc, Rat
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Classe de qualité
- Verified
- Isotype
- IgG
- Top Product
- Discover our top product VIM Anticorps primaire
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- Indications d'application
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Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 5 μg/mL.
Western Blot: Approx 55 kDa band observed in lysates of cell line HeLa and Jurkat and in Mouse Ovary lysates, and approx. 55-60 kDa band in Rat Ovary lysates (calculated MW of 53.7 kDa according to Human NP_003371.2, Mouse NP_035831.2 and Rat NP_112402.1).
Peptide ELISA: antibody detection limit dilution 1:4000.
- Commentaires
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Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml. Immunofluorescence: Strong expression of the protein seen in the cytoplasm/Intermediate filaments of U2OS cells. Recommended conc
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Minimize freezing and thawing.
- Stock
- -20 °C
- Stockage commentaire
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Antigène
- Vimentin (VIM)
- Autre désignation
- VIM (VIM Produits)
- Synonymes
- anticorps CTRCT30, anticorps cb28, anticorps vime, anticorps vim, anticorps vim1, anticorps vim2, anticorps VIM, anticorps Vimentin, anticorps vim4, anticorps vimentin, anticorps vimentin L homeolog, anticorps vimentin S homeolog, anticorps VIM, anticorps Vim, anticorps vim, anticorps vim.L, anticorps vim.S
- Sujet
- VIM, vimentin, FLJ36605
- ID gène
- 7431, 22352, 81818
- NCBI Accession
- NP_003371
- Pathways
- Caspase Cascade in Apoptosis
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