Flow Cytometry. Monitoring of subsets in peripheral blood. Characterization of leukemias and lymphomas. Analysis of CTL-mediated cytotoxicity. Studies of transplantation rejection. Functional studies of cell adhesion molecules. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Protocole
PBMC: Add 10 ul of MAB/10e6 PBMC in 100 ul PBS. Mix gently and incubate for 15 minutes at 2-8°C. Wash twice with PBS and analyze or fix with 0. 5% v/v of paraformaldehyde in PBS andanalyze. WHOLE BLOOD: Add 10 ul of MAB/100 ul of whole blood. Mix gently and incubate for 15 minutes at room temperature (20°C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0. 5% v/v of paraformaldehyde in PBS andanalyze. See instrument manufacturers instructions for Lysed Whole Blood andImmunofluorescence analysis with a flow cytometer or microscope.
Restrictions
For Research Use only
Buffer
Sterile filtered PBS containing 0.2 % protein carrier and 0.08 % Sodium Azide as preservative
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C
Stockage commentaire
Store the antibody undiluted at 2-8 °C. Do Not Freeze! Shelf life: One year from despatch.