NAPA anticorps
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- Antigène Voir toutes NAPA Anticorps
- NAPA (N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA))
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Reactivité
- Humain, Rat, Boeuf (Vache)
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp NAPA est non-conjugé
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Application
- Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)
- Specificité
- This antibody specifically recognizes a-SNAP as a single band of ~36 kDa on western blot of rat kidney, rat brain and MDBK cells and do not recognize b-SNAP.
- Attributs du produit
- Synonyms: NAPA, SNAP-alpha, SNAPA, Alpha-soluble NSF attachment protein,N-ethylmaleimide-sensitive factor attachment protein alpha
- Purification
- Standard chromatographic techniques.
- Immunogène
- Hybridoma produced by the fusion of splenocytes from mice immunized with recombinanthuman a-SNAP protein and mouse myeloma cells.
- Clone
- 15D4
- Isotype
- IgG1
- Top Product
- Discover our top product NAPA Anticorps primaire
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- Indications d'application
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Western Blot: 0.5-5 μg/mL. Immunoprecipitationon native and denatured samples: 2-10 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Buffer
- 20 mM Sodium Phosphate, pH 7.5, 150 mM Sodium Chloride, 50 % Glycerol without preservatives.
- Agent conservateur
- Without preservative
- Stock
- -20 °C
- Stockage commentaire
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Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch. - Date de péremption
- 12 months
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- Antigène
- NAPA (N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA))
- Autre désignation
- SNAP alpha (NAPA Produits)
- Synonymes
- anticorps SNAPA, anticorps wu:fa26c04, anticorps zgc:63906, anticorps napb, anticorps 1500039N14Rik, anticorps AW209189, anticorps RA81, anticorps SNARE, anticorps a-SNAP, anticorps hyh, anticorps alpha-SNAP, anticorps Alpha-SNAP, anticorps NSF attachment protein alpha, anticorps N-ethylmaleimide-sensitive factor attachment protein, alpha b, anticorps NSF attachment protein alpha L homeolog, anticorps N-ethylmaleimide-sensitive factor attachment protein, alpha, anticorps N-ethylmaleimide sensitive fusion protein attachment protein alpha, anticorps soluble NSF attachment protein, anticorps NAPA, anticorps napab, anticorps napa.L, anticorps napa, anticorps EHI_000300, anticorps Napa, anticorps LOC102577508
- Sujet
- SNAPs (soluble NSF attachment proteins), acting in concert with SNAREs (SNAP receptors) and the N-ethylmaleimide-sensitive fusion protein (NSF), are required for the fusion of transport vesicles to their target membranes in synaptic transmission, intra-Golgi transport, endosome-to-endosome fusion and transcytotic vesicles-to-plasma membrane transport. Vesicle-to-target membrane docking (initial contact) occurs when the vesicle SNARE binds to its cognate target membrane SNARE. a-SNAP (or bSNAP in brain) then binds to this docking complex and mediates the binding of NSF and thus the formation of a 20 S fusion particle. It is thought that, once NSF is bound, ATP hydrolysis by NSF initiates the fusion process. Whereas a-SNAP is expressed in all mammalian tissues, b-SNAP is expressed only in brain.Synonyms: Alpha-soluble NSF attachment protein, N-ethylmaleimide-sensitive factor attachment protein alpha, NAPA, SNAP-alpha, SNAPA
- ID gène
- 8775
- UniProt
- P54920
- Pathways
- Synaptic Vesicle Exocytosis, Asymmetric Protein Localization
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