Recognizes in Western blotting a doublet of 52-56 kDa, identified as serine-phosphorylated and unphosphorylated forms of melanocytic isoforms of microphthalmia (Mi) transcription factor. This antibody does not cross-react with other b-HLH-ZIP factors by DNA mobility shift assay.does not
Hybridoma produced by the fusion of splenocytes from RBF/DnJ mice immunized with anN-terminal fragment of human microphthalmia protein and mouse myeloma NS1 cells. Genename: MITF
Gel Supershift. Western Blot (1 μg/mL). Immunoprecipitation (2 μg/mg of protein lysate). Immunohistochemistry (Frozen and Paraffin Sections). Positive Control: 501 Mel human melanoma cells, wild-type human, rat, mouse osteoclastcells. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS containing 0.08 % Sodium Azide as preservative
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
-20 °C
Stockage commentaire
Store the antibody at -20 °C. Avoid repeated freezing and thawing. Shelf life: One year from despatch.
Date de péremption
12 months
Gehlsen, Szaszák, Gebert, Koop, Hüttmann, Steven: "Non-Invasive Multi-Dimensional Two-Photon Microscopy enables optical fingerprinting (TPOF) of immune cells." dans: Journal of biophotonics, Vol. 8, Issue 6, pp. 466-79, (2016) (PubMed).
Hägglund, Berghard, Carlsson: "Canonical Wnt/?-catenin signalling is essential for optic cup formation." dans: PLoS ONE, Vol. 8, Issue 12, pp. e81158, (2013) (PubMed).
Yamada, Ohno, Aoki, Semi, Watanabe, Moritake, Shiozawa, Kunisada, Kobayashi, Toguchida, Shimizu, Hara, Yamada: "EWS/ATF1 expression induces sarcomas from neural crest-derived cells in mice." dans: The Journal of clinical investigation, Vol. 123, Issue 2, pp. 600-10, (2013) (PubMed).
Nakano, Ando, Takata, Kawada, Muguruma, Sekiguchi, Saito, Yonemura, Eiraku, Sasai: "Self-formation of optic cups and storable stratified neural retina from human ESCs." dans: Cell stem cell, Vol. 10, Issue 6, pp. 771-85, (2012) (PubMed).
There are two known isoforms of MiTF differing by 66 amino acids at the NH2 terminus. Shorter forms are expressed in melanocytes and run as two bands at 52 kDa and 56 kDa, while the longer Mi form runs as a cluster of bands at 60-70 kDa in osteoclasts and in B16 melonoma cells (but not other melanoma cell lines), as well as mast cells and heart. It reacts with both melanocytic as well as the non- melanocytic isoforms of MiTF. Mi is a basic helix-loop-helix-leucin zipper (b-HLH-ZIP) transtripotion factor implicated in pigmentation, mast cells and bone development. The mutation of MiTF causes Waardenburg Syndrome type II in humans. In mice, a profound loss of pigmented cells in the skin eye and inner ear results, as well as osteopetrosis and defects in natural killer and mast cells. These melanocyte isoforms have been shown by two dimensional tryptic mapping to differ in c-Kit-induced phosphorylation. Osteopetrotic rat strain harbors a large genomic deletion encompassing the 3Synonyms: Mi-protein, Microphthalmia-associated transcription factor