FA2H anticorps
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- Antigène Voir toutes FA2H Anticorps
- FA2H (Fatty Acid 2-Hydroxylase (FA2H))
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Reactivité
- Humain
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp FA2H est non-conjugé
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Application
- Western Blotting (WB)
- Specificité
- This antibody recognizes Fatty Acid 2-hydrolase (FA2H).
- Purification
- Purified
- Immunogène
- Synthetic peptide derived from Human fatty acid 2-hydrolase (FA2H) protein.
- Top Product
- Discover our top product FA2H Anticorps primaire
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- Indications d'application
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ELISA. Western Blot: 5-10 μg/mL. Positive Control: Pancreas.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Buffer
- Phosphate buffered saline with 0.08 % Sodium Azide as preservative.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Avoid repeated freezing and thawing.
- Stock
- -20 °C
- Stockage commentaire
- The antibody can be shipped at ambient temperature. Store (in aliquots) at -20 °C only.
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- Antigène
- FA2H (Fatty Acid 2-Hydroxylase (FA2H))
- Autre désignation
- Fatty Acid alpha-Hydroxylase (FA2H Produits)
- Synonymes
- anticorps FAAH, anticorps FAH1, anticorps FAXDC1, anticorps SCS7, anticorps SPG35, anticorps RGD1310347, anticorps Wdr59, anticorps Faxdc1, anticorps G630055L08Rik, anticorps MGC85470, anticorps MGC97767, anticorps cb1044, anticorps zgc:153777, anticorps fatty acid 2-hydroxylase, anticorps fatty acid 2-hydroxylase L homeolog, anticorps fatty acid hydroxylase, anticorps FA2H, anticorps Fa2h, anticorps fa2h.L, anticorps fa2h, anticorps Tsp_11648, anticorps Ilyop_0703, anticorps Celly_1453, anticorps Weevi_1515, anticorps Fluta_1455, anticorps Mesop_3301
- Sujet
- 2-Hydroxysphingolipids are a subset of sphingolipids containing 2-hydroxy fatty acids. The 2-hydroxylation occurs during de novo ceramide synthesis and is catalyzed by fatty acid 2-hydroxylase (also known as fatty acid alpha-hydroxylase). In mammals, 2-hydroxysphingolipids are present abundantly in brain because the major myelin lipids galactosylceramides and sulfatides contain 2-hydroxy fatty acids. Here we report identification and characterization of a human gene that encodes a fatty acid 2-hydroxylase. Data base searches revealed a human homologue of the yeast ceramide 2-hydroxylase gene (FAH1), which we named FA2H. The FA2H gene encodes a 372 - amino acid protein with 36 % identity and 46 % similarity to yeast Fah1p. The amino acid sequence indicates that FA2H protein contains an N-terminal cytochrome b5 domain and four potential transmembrane domains. FA2H also contains the iron-binding histidine motif conserved among membrane-bound desaturases/hydroxylases. COS7 cells expressing human FA2H contained 3-20-fold higher levels of 2-hydroxyceramides (C16, C18, C24, and C24:1) and 2-hydroxy fatty acids compared with control cells. Microsomal fractions prepared from transfected COS7 cells showed tetracosanoic acid 2-hydroxylase activities in an NADPH- and NADPH: cytochrome P-450 reductase-dependent manner. FA2H lacking the N-terminal cytochrome b5 domain had little activity.Synonyms: FA2H, FAAH, Fatty acid 2-hydroxylase
- ID gène
- 79152
- NCBI Accession
- NP_077282
- UniProt
- Q7L5A8
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