Involucrin anticorps
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- Antigène Voir toutes Involucrin (IVL) Anticorps
- Involucrin (IVL)
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Reactivité
- Humain
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp Involucrin est non-conjugé
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
- Specificité
- This antibody detects endogenous levels of Involucrin protein. (region surrounding Pro568)
- Réactivité croisée (Details)
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. - Purification
- Affinity Chromatography using epitope-specific immunogen.
- Top Product
- Discover our top product IVL Anticorps primaire
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- Indications d'application
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ELISA: 1/10000approx. 1/20000. Western Blot: 1/500approx. 1/1000. Immunohistochemistry: 1/50approx. 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Avoid repeated freezing and thawing.
- Stock
- 4 °C/-20 °C
- Stockage commentaire
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Antigène
- Involucrin (IVL)
- Autre désignation
- Involucrin / IVL (IVL Produits)
- Synonymes
- anticorps INV, anticorps NPH2, anticorps NPHP2, anticorps 1110019C06Rik, anticorps IVL, anticorps Nphp2, anticorps inv, anticorps involucrin, anticorps inversin, anticorps IVL, anticorps INVS, anticorps Ivl, anticorps Invs
- Sujet
- Involucrin is a precursor protein of the keratinocyte cornified envelope, which is formed beneath the inner surface of the cell membrane during terminal differentiation. Involucrin first appears in the cell cytosol but ultimately becomes cross-linked to membrane proteins by transglutaminase. During keratinocyte terminal differentiation glutamine residues of involucrin become covalently cross-linked to other envelope precursors via covalent ε-(γ-glutamyl) lysine bonds. Moreover, its large size allows involucrin to cross-link molecules that are separated by substantial distances in the cornified envelope. These properties allow a single involucrin molecule to form multiple cross-links, in multiple spatial planes, with other envelope precursors. Involucrin is specifically expressed in Chinese hamster ovarian cells (fibroblasts), PtK2 rat kangaroo kidney cells (simple epithelial) and rat epidermal keratinocytes (stratifying squamous epithelial).
- Poids moléculaire
- approx. 68 kDa
- ID gène
- 3713
- NCBI Accession
- NP_005538
- UniProt
- P07476
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