Cet anticorp Asialoglycoprotein Receptor 1 est non-conjugé
Application
Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), BioImaging (BI)
Marque
BD Pharmingen™
Attributs du produit
The 8D7 monoclonal antibody recognizes Asialoglycoprotein receptor 1 (ASGPR 1), also known as Hepatic lectin H1 (HL-1). ASGPR 1 is an approximately 42 kDA type II integral membrane protein that is expressed on the surface of hepatic cells. It is expressed by hepatocytes on the sinusoidal-lateral plasma membrane but not on the bile canalicular membrane. ASGPR 1 plays a role in serum glycoprotein homeostasis. It functions as a subunit of the Asialoglycoprotein receptor (ASGPR) complex that binds, internalizes, and transports various glycoproteins for lysosomal degradation. The receptor may also promote hepatic infection by the binding and uptake of various viruses. The immunogen used to generate the 8D7 hybridoma was rat liver membrane extracts. Rat ASGPR consists of three polypeptide subunits (Rat hepatic lectin 1-3 (RHL1-3). The 8D7 antibody has been shown to react with a subunit-specific epitope on RHL-1.
Flow Cytometry System. Immunofluorescent Staining of ASGPR 1 in Human Hepatocellular Carcinoma (Hep G2) cells. Hep G2 cells (ATCC, HB-8065) were fixed with BD Cytofix™ Fixation Buffer and stained with Purified Mouse Anti-ASGPR 1 (pseudo-colored green) at 5 μg/mL. The second-step reagent was Alexa Fluor® 488 Goat Anti-Mouse IgG (Life Technologies), and counter-staining was with DAPI (4',6-Diamidino-2-phenylindole dihydrochloride, pseudo-colored blue). The images were captured on a BD Pathway™ 435 Cell Analyzer and merged using BD AttoVision™ Software.
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C
Stockage commentaire
Store undiluted at 4°C.
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