CD28 anticorps
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- Antigène Voir toutes CD28 Anticorps
- CD28
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Reactivité
- Rat
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp CD28 est non-conjugé
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Application
- Flow Cytometry (FACS), Immunoprecipitation (IP)
- Marque
- BD Pharmingen™
- Attributs du produit
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The JJ319 antibody reacts with CD28, which is expressed on virtually all T lymphocytes bearing αβ T-cell receptors (TCR), on most γδ TCR-bearing T cells, and on a subset of NK cells. In the thymus, CD28 expression is developmentally regulated during the maturation of αβ TCR-bearing T cells. CD28 is a costimulatory receptor required for activation of T cells, its ligands include CD80 (B7-1) and CD86 (B7-2). Soluble JJ319 mAb costimulates the proliferative responses and IL-2 production of CD4+ and CD8+ T cells activated by anti-αβ-TCR mAb R73 (Cat. no. 554910). The alternate anti-rat CD28 mAb JJ316 (Cat. No. 554992) is capable of directly stimulating T cells in vitro and in vivo. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Two-color analysis of the expression of CD28 in rat thymus. LEW thymocytes were simultaneously stained with FITC-conjugated anti-rat CD3 mAb G4.18 (Cat. No. 559975/554832) and purified mAb JJ319 (right panel), followed by PE-conjugated anti-mouse IgG1 mAb A85-1 (Cat. No. 550083). Flow cytometry was performed on a BD FACScan™ flow cytometry system.
BD Pharmingen™ Purified Mouse Anti-Rat CD28 - Purified - Clone JJ319 - Isotype Mouse IgG1, κ - Reactivity Rat - 0.1 mg - Purification
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Immunogène
- Rat CD28-transfected cell line
- Clone
- JJ319
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product CD28 Anticorps primaire
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- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Concentration
- 0.5 mg/mL
- Buffer
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C
- Stockage commentaire
- Store undiluted at 4°C.
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CD28-mediated induction of proliferation in resting T cells in vitro and in vivo without engagement of the T cell receptor: evidence for functionally distinct forms of CD28." dans: European journal of immunology, Vol. 27, Issue 1, pp. 239-47, (1997) (PubMed).
: "New perspectives of CD28-B7-mediated T cell costimulation." dans: Immunity, Vol. 2, Issue 6, pp. 555-9, (1995) (PubMed).
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CD28-mediated induction of proliferation in resting T cells in vitro and in vivo without engagement of the T cell receptor: evidence for functionally distinct forms of CD28." dans: European journal of immunology, Vol. 27, Issue 1, pp. 239-47, (1997) (PubMed).
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- Antigène
- CD28
- Autre désignation
- CD28 (CD28 Produits)
- Synonymes
- anticorps Tp44, anticorps CD28RNA, anticorps cd28, anticorps CD28 molecule, anticorps CD28 antigen, anticorps Cd28 molecule, anticorps CD28 molecule L homeolog, anticorps CD28, anticorps Cd28, anticorps cd28.L
- Pathways
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
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