Monoclonal antibody BM16 reacts with CRTH2 (chemoattractant receptor-homologous molecule expressed on Th2 cells). CRTH2 is a member of the G protein-coupled leukocyte chemoattractant receptor family and it is expressed on Th2, but not Th1 lineage cells. It is detectable on CD4+ T cells in fresh PBMC and no significant expression on B cells and NK cells. CRTH2 is also expressed on peripheral blood basophils and eosinophils, suggesting its involvement allergic reactions. Phenotypic analysis of CD4+ T cells expressing CRTH2 demonstrated CD45RA-, CD45RO+ and CD25+. These cells produce Th2- but little or no Th1-type cytokines upon stimulation with PMA and Ionomycin. Profile of anti-CRTH2 (BM16) reactivity on HUT 78 cells analyzed by flow cytometry. Second step staining with Cat. No. 554016.
BD Pharmingen™ Purified Rat Anti-Human CD294 - Purified - Clone BM16 - Isotype Rat IgG2a, κ - Reactivity Hu - 0.1 mg
Purification
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C
Stockage commentaire
Store undiluted at 4°C.
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Nagata, Hirai, Tanaka, Ogawa, Aso, Sugamura, Nakamura, Takano: "CRTH2, an orphan receptor of T-helper-2-cells, is expressed on basophils and eosinophils and responds to mast cell-derived factor(s)." dans: FEBS letters, Vol. 459, Issue 2, pp. 195-9, (1999) (PubMed).
Nagata, Tanaka, Ogawa, Kemmotsu, Imai, Yoshie, Abe, Tada, Nakamura, Sugamura, Takano: "Selective expression of a novel surface molecule by human Th2 cells in vivo." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 3, pp. 1278-86, (1999) (PubMed).