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- Antigène Voir toutes CD4 Anticorps
- CD4
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Reactivité
- Souris
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Hôte
- Rat
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp CD4 est non-conjugé
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Application
- Flow Cytometry (FACS)
- Marque
- BD Pharmingen™
- Attributs du produit
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The RM4-4 antibody reacts with the CD4 (L3T4) differentiation antigen expressed on most thymocytes, a subpopulation of mature T lymphocytes (i.e., MHC class II-restricted T cells, including most T helper cells), and a subset of NK-T cells of all mouse strains tested. CD4 has also been detected at low density on pluripotent hematopoietic stem cells, bone marrow myeloid and B-lymphocyte precursors, intrathymic lymphoid precursors, and a subset of splenic dendritic cells. CD4 is expressed on the plasma membrane of mouse egg cells and is involved in adhesion of the egg to MHC class II-bearing sperm. CD4 is an antigen coreceptor on the T-cell surface which interacts with MHC class II molecules on antigen-presenting cells. It participates in T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase lck. Purified RM4-4 mAb does not block binding of FITC-conjugated GK1.5 mAb (Cat. No. 557307/553729), H129.19 mAb (Cat. No. 553650/553651), or RM4-5 mAb (Cat. No. 553046/553047) to T cells. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.
BD Pharmingen™ Purified Rat Anti-Mouse CD4 - Purified - Clone RM4-4 - Isotype Rat IgG2b, κ - Reactivity Ms - 0.5 mg - Purification
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Immunogène
- BALB/c mouse thymocytes
- Clone
- RM4
- Isotype
- IgG2b kappa
- Top Product
- Discover our top product CD4 Anticorps primaire
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- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Concentration
- 0.5 mg/mL
- Buffer
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C
- Stockage commentaire
- Store undiluted at 4°C.
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Concept of lymphoid versus myeloid dendritic cell lineages revisited: both CD8alpha(-) and CD8alpha(+) dendritic cells are generated from CD4(low) lymphoid-committed precursors." dans: Blood, Vol. 96, Issue 7, pp. 2511-9, (2000) (PubMed).
: "Commitment to the B lymphoid lineage occurs before DH-JH recombination." dans: The Journal of experimental medicine, Vol. 189, Issue 4, pp. 735-40, (1999) (PubMed).
: "Mouse NK1+ T cells." dans: Current opinion in immunology, Vol. 7, Issue 3, pp. 367-74, (1995) (PubMed).
: "Molecular structure and function of CD4 on murine egg plasma membrane." dans: Zygote (Cambridge, England), Vol. 3, Issue 1, pp. 65-73, (1995) (PubMed).
: "Onset of TCR-beta gene rearrangement and role of TCR-beta expression during CD3-CD4-CD8- thymocyte differentiation." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 10, pp. 4783-92, (1994) (PubMed).
: "The T cell receptor as a multicomponent signalling machine: CD4/CD8 coreceptors and CD45 in T cell activation." dans: Annual review of immunology, Vol. 10, pp. 645-74, (1992) (PubMed).
: "CD4 is expressed on murine pluripotent hematopoietic stem cells." dans: Blood, Vol. 80, Issue 7, pp. 1717-24, (1992) (PubMed).
: "Developmental potential of the earliest precursor cells from the adult mouse thymus." dans: The Journal of experimental medicine, Vol. 174, Issue 6, pp. 1617-27, (1992) (PubMed).
: "CD4 expressed on earliest T-lineage precursor cells in the adult murine thymus." dans: Nature, Vol. 349, Issue 6304, pp. 71-4, (1991) (PubMed).
: "The biologic roles of CD2, CD4, and CD8 in T-cell activation." dans: Annual review of immunology, Vol. 7, pp. 579-99, (1989) (PubMed).
: "L3T4 antigen expression by hemopoietic precursor cells." dans: The Journal of experimental medicine, Vol. 169, Issue 4, pp. 1473-8, (1989) (PubMed).
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Concept of lymphoid versus myeloid dendritic cell lineages revisited: both CD8alpha(-) and CD8alpha(+) dendritic cells are generated from CD4(low) lymphoid-committed precursors." dans: Blood, Vol. 96, Issue 7, pp. 2511-9, (2000) (PubMed).
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- Antigène
- CD4
- Autre désignation
- CD4 (CD4 Produits)
- Synonymes
- anticorps cd4, anticorps L3T4, anticorps Ly-4, anticorps CD4mut, anticorps W3/25, anticorps p55, anticorps CD4 molecule, anticorps carcinoembryonic antigen-related cell adhesion molecule 5, anticorps T-cell surface glycoprotein CD4, anticorps CD4 antigen, anticorps Cd4 molecule, anticorps CD4, anticorps LOC100125525, anticorps cd4, anticorps Cd4
- Sujet
- Synonyms: L3T4
- Pathways
- TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events
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