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- Antigène Voir toutes CD4 Anticorps
- CD4
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Reactivité
- Rat
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp CD4 est conjugé à/à la Biotin
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Application
- Flow Cytometry (FACS)
- Marque
- BD Pharmingen™
- Attributs du produit
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The OX-35 clone has been reported to react with the CD4 antigen on most thymocytes, a subpopulation of mature T lymphocytes (i.e. MHC class II-restricted T cells, including most T helper cells), monocytes, macrophages, some dendritic cells, and microglia. CD4 is an antigen coreceptor on the T-cell surface that interacts with MHC class II molecules on antigen-presenting cells. It participates in T-cell activation through it's association with the T-cell receptor complex and protein tyrosine kinase lck. The OX-35 clone has been reported to bind to a different epitope of CD4 than that recognized by the W3/25 and OX-38 clones. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Two-color analysis of the expression of CD4 on rat splenic leukocytes. Lewis splenocytes were simultaneously stained with biotinylated anti-rat CD4 mAb clone OX-35 (right panel) and PE conjugated anti-rat CD3 mAb clone G4.18 (Cat. No. 554833), followed by avidin-FITC (Cat. No. 554057). The CD3-negative CD4-dim cells are the monocyte/macrophage population. Flow cytometry was performed on a BD FACScan™ flow cytometry system.
BD Pharmingen™ Biotin Mouse Anti-Rat CD4 - Biotin - Clone OX-35 - Isotype Mouse IgG2a, κ - Reactivity Rat - 0.5 mg - Purification
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Immunogène
- Rat T-cell blasts
- Clone
- OX
- Isotype
- IgG2a kappa
- Top Product
- Discover our top product CD4 Anticorps primaire
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- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Concentration
- 0.5 mg/mL
- Buffer
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
- Stock
- 4 °C
- Stockage commentaire
- Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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Dendritic cell heterogeneity in vivo: two functionally different dendritic cell populations in rat intestinal lymph can be distinguished by CD4 expression." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 3, pp. 1146-55, (1998) (PubMed).
: "Immunohistochemical study of amoeboid microglial cells in fetal rat brain." dans: Journal of anatomy, Vol. 189 ( Pt 3), pp. 567-74, (1997) (PubMed).
: "Tissue digestion with dispase substantially reduces lymphocyte and macrophage cell-surface antigen expression." dans: Journal of immunological methods, Vol. 194, Issue 1, pp. 71-5, (1996) (PubMed).
: "Cell-surface marker analysis of rat thymic dendritic cells." dans: Immunology, Vol. 79, Issue 2, pp. 298-304, (1993) (PubMed).
: "The T cell receptor as a multicomponent signalling machine: CD4/CD8 coreceptors and CD45 in T cell activation." dans: Annual review of immunology, Vol. 10, pp. 645-74, (1992) (PubMed).
: "The biologic roles of CD2, CD4, and CD8 in T-cell activation." dans: Annual review of immunology, Vol. 7, pp. 579-99, (1989) (PubMed).
: "Authentic T helper CD4 (W3/25) antigen on rat peritoneal macrophages." dans: The Journal of experimental medicine, Vol. 162, Issue 1, pp. 117-27, (1985) (PubMed).
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Dendritic cell heterogeneity in vivo: two functionally different dendritic cell populations in rat intestinal lymph can be distinguished by CD4 expression." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 3, pp. 1146-55, (1998) (PubMed).
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- Antigène
- CD4
- Autre désignation
- CD4 (CD4 Produits)
- Synonymes
- anticorps cd4, anticorps L3T4, anticorps Ly-4, anticorps CD4mut, anticorps W3/25, anticorps p55, anticorps CD4 molecule, anticorps carcinoembryonic antigen-related cell adhesion molecule 5, anticorps T-cell surface glycoprotein CD4, anticorps CD4 antigen, anticorps Cd4 molecule, anticorps CD4, anticorps LOC100125525, anticorps cd4, anticorps Cd4
- Pathways
- TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events
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