Cet anticorp p53 est conjugé à/à la Alexa Fluor 647
Application
Intracellular Staining (ICS)
Marque
BD Phosflow™
Attributs du produit
The p53 protein is critical to regulation of normal cell growth and proliferation and is a suppressor of tumor cell proliferation. Inactivation of p53 by a number of mechanisms, such as missense mutations or interaction with oncogenic viral or cellular proteins, can result in tumor progression. Mutations and/or allelic loss of the p53 gene are associated with a wide variety of human tumors. Known to have a role in transcriptional regulation, p53 suppresses various promoters containing TATA elements in an apparently sequence-independent fashion. p53 also binds to DNA in a sequence-specific manner via recognition of a 20-bp consensus-binding site. This interaction stimulates the expression of genes downstream of the p53 binding site. A number of genes that contain p53-binding sites have been identified, including MDM2, GADD45, and muscle creatine kinase. Post-translational acetylation of p53 enhances its DNA-binding activity. There are multiple factors that affect p53 acetylation, thereby modulating cellular proliferation and apoptosis. The L82-51 monoclonal antibody recognizes acetylated lysine 382 (acK382) in the C-terminal region of p53. Analysis of p53 (acK382) in lymphocytes. Human peripheral blood mononuclear cells (PBMC) were either treated with 0.4 μM Trichostatin A (Sigma, Cat. No. T8552) plus 0.4 μM Adriamycin (Doxorubicin hydrochloride, Sigma, Cat. No. D1515) for 24 hours (shaded histogram) or untreated (open histogram). The cells were fixed (BD Cytofix™ buffer, Cat. No. 554655) for 10 minutes, then permeabilized (BD Phosflow™ Perm Buffer III, Cat. No. 558050) on ice for at least 30 minutes, and then stained with Alexa Fluor® 647 Mouse anti-p53 (acK382). Lymphocytes were selected by scatter profile. Flow cytometry was performed on a BD FACSArray™ bioanalyzer system. The specificity of mAb L82-51 was confirmed by western blot analysis using unconjugated antibody on lysates from control (left blot) and Trichostatin A-plus-Adriamycin-treated (right blot) PBMC. p53 (acK382) is identified as a band of 53 kDa in the treated cells.
TP53
Reactivité: Humain, Souris, Rat
WB, ELISA, IHC, IF, ICC
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
Optimal working dilution should be determined by the investigator.
Volume d'échantillon
20 μL
Restrictions
For Research Use only
Buffer
Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
Stock
4 °C
Stockage commentaire
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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