DCP1A anticorps

N° du produit ABIN272254

Détail du produit anti-DCP1A anticorps

Antigène: DCP1A (DCP1 Decapping Enzyme Homolog A (S. Cerevisiae) (DCP1A))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp DCP1A est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificité: This antibody detects endogenous levels of SMIF protein. (region surrounding Ser133)

Réactivité croisée (Details): Species reactivity (tested):Human, Mouse.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Pureté: > 95 % pure by SDS-PAGE

Information d'application

Indications d'application: ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: DO NOT FREEZE!

Stock: 4 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C.

Détails sur DCP1A

Antigène: DCP1A (DCP1 Decapping Enzyme Homolog A (S. Cerevisiae) (DCP1A))

Autre désignation: DCP1A (DCP1A Produits)

Synonymes: anticorps DCP1A, anticorps 1110066A22Rik, anticorps 4930568L04Rik, anticorps AU019772, anticorps D14Ertd817e, anticorps Mitc1, anticorps SMIF, anticorps RGD1561298, anticorps mitc1, anticorps HSA275986, anticorps Nbla00360, anticorps SMAD4IP1, anticorps smad4ip1, anticorps smif, anticorps decapping mRNA 1A, anticorps decapping mRNA 1A L homeolog, anticorps DCP1A, anticorps Dcp1a, anticorps dcp1a, anticorps dcp1a.L

Sujet: Signal transduction of TGF-beta superfamily members is regulated by Smad proteins. In particular, Smads influence specific gene transcription by relaying signals from the cell membrane to the nucleus. Smad4 plays an essential role in TGF-beta-induced transcriptional activation wherein phosphorylated receptor-associated Smads associate with Smad4. Furthermore, SMIF (Smad4-intereacting protein) and Smad4 complex with TGF-beta and BMP4. An increase in Smad4 concentration increases the translocation of this complex to the nucleus. SMIF and Smad4 interact directly through a EVH1/WH1 domain on SMIF and a proline-rich activation domain on Smad4. Smad4 is essential to nuclear translocation of SMIF as deletion of the Smad4-interacting domain (located in the N-terminal 100 amino acids) of SMIF eliminates TGF-β-induced nuclear translocation of SMIF (1). The human SMIF gene is ubiquitously expressed and encodes a protein with a relative molecular mass of 70 kDa.Synonyms: SMIF, Smad4-interacting transcriptional co-activator, Transcription factor SMIF, mRNA-decapping enzyme 1A

Poids moléculaire: approx. 75 kDa

ID gène: 55802

NCBI Accession: NP_060873

UniProt: Q9NPI6