LGR5 anticorps (AA 250-550)

N° du produit ABIN2724692

Détail du produit anti-LGR5 anticorps

Antigène: LGR5 (Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5 (LGR5))

Épitope: AA 250-550

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp LGR5 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Attributs du produit: Homo sapiens leucine-rich repeat containing G protein-coupled receptor 5 (LGR5)

Purification: Purified from mouse ascites fluids by affinity chromatography

Immunogène: Human recombinant protein fragment corresponding to amino acids 250-550 of human LGR5 (NP_003658) produced in HEK293T Cell.

Clone: 2A2

Isotype: IgG1

Information d'application

Indications d'application: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100, IP 2 μg/500 μL

Commentaires:

The concentration of the product may vary between diferrent lots.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5-1.0 mg/mL

Buffer: PBS (PH 7.3) containing 1 % BSA, 50 % glycerol and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Références pour anticorps anti-LGR5 (ABIN2724692)

Patel, Sharon, Baranwal, Boothello, Desai, Patel: "Heparan sulfate hexasaccharide selectively inhibits cancer stem cells self-renewal by activating p38 MAP kinase." dans: Oncotarget, Vol. 7, Issue 51, pp. 84608-84622, (2018) (PubMed).

Yang, Bavi, Wang, Roehrl: "Immuno-proteomic discovery of tumor tissue autoantigens identifies olfactomedin 4, CD11b, and integrin alpha-2 as markers of colorectal cancer with liver metastases." dans: Journal of proteomics, Vol. 168, pp. 53-65, (2018) (PubMed).

Miyake, Li, Lee, Koike, Chen, Seo, Pierro: "Liver damage, proliferation, and progenitor cell markers in experimental necrotizing enterocolitis." dans: Journal of pediatric surgery, Vol. 53, Issue 5, pp. 909-913, (2018) (PubMed).

Ma, Wang, Wang, Luo, Fan: "NANOGP8 is the key regulator of stemness, EMT, Wnt pathway, chemoresistance, and other malignant phenotypes in gastric cancer cells." dans: PLoS ONE, Vol. 13, Issue 4, pp. e0192436, (2018) (PubMed).

Liu, Zheng, Ji, Liu, Hou, Li, Li: "Resveratrol reduces senescence-associated secretory phenotype by SIRT1/NF-κB pathway in gut of the annual fish Nothobranchius guentheri." dans: Fish & shellfish immunology, Vol. 80, pp. 473-479, (2018) (PubMed).

Wang, Chen, Cao, Ma, Yin, Jia, Zang, Fan: "LGR5 Is a Gastric Cancer Stem Cell Marker Associated with Stemness and the EMT Signature Genes NANOG, NANOGP8, PRRX1, TWIST1, and BMI1." dans: PLoS ONE, Vol. 11, Issue 12, pp. e0168904, (2017) (PubMed).

Osawa, Takahashi, Nishimura, Ohta, Haraguchi, Hata, Yamamoto, Mizushima, Takemasa, Doki, Mori: "Full-length LGR5-positive cells have chemoresistant characteristics in colorectal cancer." dans: British journal of cancer, Vol. 114, Issue 11, pp. 1251-60, (2016) (PubMed).

Chen, Wei, Sun, Bhattacharya, Savage, Serda, Mackeyev, Curley, Bu, Wang, Chen, Cohen-Gould, Huang, Shen, Lipkin, Copeland, Jenkins, Shuler: "A recellularized human colon model identifies cancer driver genes." dans: Nature biotechnology, Vol. 34, Issue 8, pp. 845-51, (2016) (PubMed).

Hsu, Liu, Tseng, Yang, Yeh, You, Hung, Chen, Chen: "CBB1003, a lysine-specific demethylase 1 inhibitor, suppresses colorectal cancer cells growth through down-regulation of leucine-rich repeat-containing G-protein-coupled receptor 5 expression." dans: Journal of cancer research and clinical oncology, Vol. 141, Issue 1, pp. 11-21, (2015) (PubMed).

Shaikh, Zhou, Teo, Garg, Neogi, Figg, Yeo, Yu, Maguire, Zhao, Bennett, Azizan, Davenport, McKenzie, Brown: "LGR5 Activates Noncanonical Wnt Signaling and Inhibits Aldosterone Production in the Human Adrenal." dans: The Journal of clinical endocrinology and metabolism, Vol. 100, Issue 6, pp. E836-44, (2015) (PubMed).

Jiang, Zhou, Chen, Schiff, Lee, Kong, Embree, Zhou, Mao: "Postnatal epithelium and mesenchyme stem/progenitor cells in bioengineered amelogenesis and dentinogenesis." dans: Biomaterials, Vol. 35, Issue 7, pp. 2172-80, (2014) (PubMed).

Wang, Guo, Lui, Chu, Yoo, Chang, Yen: "Identification of a distinct small cell population from human bone marrow reveals its multipotency in vivo and in vitro." dans: PLoS ONE, Vol. 9, Issue 1, pp. e85112, (2014) (PubMed).

Hsu, Liu, Tseng, Tan, Chen, Chen: "LGR5 regulates survival through mitochondria-mediated apoptosis and by targeting the Wnt/?-catenin signaling pathway in colorectal cancer cells." dans: Cellular signalling, Vol. 26, Issue 11, pp. 2333-42, (2014) (PubMed).

Lühnsdorf, Epe, Khobta: "Excision of uracil from transcribed DNA negatively affects gene expression." dans: The Journal of biological chemistry, Vol. 289, Issue 32, pp. 22008-18, (2014) (PubMed).

Wu, Xie, Gao, Wang, Guo, Tian, Li, Fan: "Lgr5 expression as stem cell marker in human gastric gland and its relatedness with other putative cancer stem cell markers." dans: Gene, Vol. 525, Issue 1, pp. 18-25, (2013) (PubMed).

Pastò, Marchesi, Diamantini, Frasson, Curtarello, Lago, Pilotto, Parenti, Esposito, Agostini, Nitti, Amadori: "PKH26 staining defines distinct subsets of normal human colon epithelial cells at different maturation stages." dans: PLoS ONE, Vol. 7, Issue 8, pp. e43379, (2012) (PubMed).

Détails sur LGR5

Antigène: LGR5 (Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5 (LGR5))

Autre désignation: LGR5 (LGR5 Produits)

Synonymes: anticorps lgr5-a, anticorps lgr5a, anticorps FEX, anticorps Gpr49, anticorps GPR49, anticorps GPR67, anticorps GRP49, anticorps HG38, anticorps leucine-rich repeat containing G protein-coupled receptor 5 L homeolog, anticorps leucine rich repeat containing G protein coupled receptor 5, anticorps leucine rich repeat containing G protein-coupled receptor 5, anticorps lgr5.L, anticorps Lgr5, anticorps LGR5

Sujet: LGR5 (leucine-rich repeat containing G protein-coupled receptor 5) An Orphan GPCR protein, contains large extracellular domain, Stem cell biomarker for small intestine, active in colon cancer, overexpressed in ovary and liver tumors.

Poids moléculaire: 99.8 kDa

ID gène: 8549

NCBI Accession: NM_003667

HGNC: 8549

Pathways: Signalisation WNT