APAF1 anticorps (N-Term)

N° du produit ABIN2745510

Détail du produit anti-APAF1 anticorps

Antigène: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Épitope: AA 1-97, N-Term

Reactivité: Humain, Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp APAF1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC)

Specificité: Recognizes the CARD domain of human and mouse Apaf-1.

Réactivité croisée: Humain, Souris

Aucune reactivité croisée: Rat (Rattus)

Réactivité croisée (Details): Does not cross-react with rat Apaf-1.

Pureté: >95 % (SDS-PAGE)

niveau d'endotoxine: <0.1EU/μg purified protein (LAL test, Lonza).

Immunogène: Recombinant mouse Apaf-1 (aa 1-97) containing the N-terminal CARD domain.

Clone: 18H2

Isotype: IgG2a

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: In PBS containing 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C.

Date de péremption: 12 months

Références pour anticorps anti-APAF1 (ABIN2745510)

Oladzad, Nikkhah, Hosseinkhani: "Optimization of Experimental Variables Influencing Apoptosome Biosensor in HEK293T Cells." dans: Sensors (Basel, Switzerland), Vol. 20, Issue 6, (2020) (PubMed).

Tashakor, H-Dehkordi, OConnell, Gomez Ganau, Gozalbes, Eriksson, Hosseinkhani, Fearnhead: "A new split-luciferase complementation assay identifies pentachlorophenol as an inhibitor of apoptosome formation." dans: FEBS open bio, Vol. 9, Issue 7, pp. 1194-1203, (2020) (PubMed).

Noori, Hosseini, Nikkhah, Hosseinkhani: "Apoptosome formation upon overexpression of native and truncated Apaf-1 in cell-free and cell-based systems." dans: Archives of biochemistry and biophysics, Vol. 642, pp. 46-51, (2019) (PubMed).

Détails sur APAF1

Antigène: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Autre désignation: Apaf-1 (APAF1 Produits)

Synonymes: anticorps APAF1, anticorps ARK, anticorps Apaf-1, anticorps Apaf1, anticorps CG6829, anticorps D-Apaf-1, anticorps DARK, anticorps Dapaf-1, anticorps Dapaf-1/HAC-1, anticorps Dark, anticorps Dark/Apaf-I, anticorps Dark/Dapaf-1/HAC1, anticorps Dark/Hac-1/dApaf-1, anticorps Dark/Hac-1/dApaf1, anticorps Dmel\\CG6829, anticorps Hac-1, anticorps Hac-1/Dark, anticorps Hac1, anticorps T1, anticorps anon-53Fa, anticorps apaf-1, anticorps apaf1, anticorps arc, anticorps ark, anticorps dAPAF-1, anticorps dApaf-1, anticorps dApaf-1/DARK/HAC-1, anticorps dApaf1, anticorps dArk, anticorps dapaf, anticorps dapaf-1, anticorps dapaf-1L, anticorps dapaf-1S, anticorps dark, anticorps dark/dapaf-1/hac-1, anticorps dark/hac-1/dapaf-1, anticorps hac-1, anticorps hac1, anticorps l(2)SH0173, anticorps APAF-1, anticorps CED4, anticorps 6230400I06Rik, anticorps Apaf1l, anticorps fog, anticorps mKIAA0413, anticorps zgc:136543, anticorps Death-associated APAF1-related killer, anticorps apoptotic peptidase activating factor 1 L homeolog, anticorps apoptotic peptidase activating factor 1, anticorps Dark, anticorps apaf1.L, anticorps apaf1, anticorps APAF1, anticorps Apaf1

Sujet: Apaf-1 is a cytoplasmic protein that forms one of the central hubs in the apoptosis regulatory network. It contains (from the N terminal) a caspase recruitment domain (CARD), an ATPase domain (NB-ARC), few short helical domains and then several copies of the WD40 repeat domain. Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome, mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis.

UniProt: O88879

Pathways: Signalisation p53, Apoptose, Caspase Cascade in Apoptosis, Tube Formation, Positive Regulation of Endopeptidase Activity