Caspase 8 anticorps

N° du produit ABIN2745525

Détail du produit anti-Caspase 8 anticorps

Antigène: Caspase 8 (CASP8)

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp Caspase 8 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Specificité: Recognizes the p20 subunit of mouse caspase-8. Detects bands of ~55 kDa (full-length caspase-8) and ~18 kDa (apoptosis-induced cleavage fragment) by Western blot.

Réactivité croisée: Souris

Aucune reactivité croisée: Humain

Réactivité croisée (Details): Does not cross-react with human caspase-8.

Pureté: >95 % (SDS-PAGE)

niveau d'endotoxine: <0.1EU/μg purified protein (LAL test, Lonza).

Immunogène: Recombinant mouse caspase-8 (p20 subunit).

Clone: 1G12

Isotype: IgG1

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: In PBS containing 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C.

Date de péremption: 12 months

Détails sur Caspase 8

Antigène: Caspase 8 (CASP8)

Autre désignation: Caspase-8 (CASP8 Produits)

Synonymes: anticorps casp8-A, anticorps xCaspase-8, anticorps casp8, anticorps CASP8, anticorps CASP-8, anticorps FLICE, anticorps MACH, anticorps Mch5, anticorps ALPS2B, anticorps CAP4, anticorps Casp-8, anticorps MCH5, anticorps caspase-8, anticorps zgc:92075, anticorps caspase 8 L homeolog, anticorps death related ced-3/Nedd2-like protein, anticorps caspase 8, anticorps caspase-8, anticorps caspase-8-like, anticorps caspase 8, apoptosis-related cysteine peptidase, anticorps casp8.L, anticorps LOC661095, anticorps CASP8, anticorps LOC5563818, anticorps casp8, anticorps LOC100222284, anticorps Casp8

Sujet: Procaspase-8 belongs to the family of caspases. Binding of FasL to Fas leads to formation of a receptor complex at the cellular membrane, which was named DISC. The DISC consists of oligomerized receptors, the DD-containing adaptor molecule FADD, procaspase-8, procaspase-10 and c-FLIP. The DISC structure provides a platform for the oligomerization of procaspase-8 that allows two procaspase-8 homodimers to be in the close proximity leading to the initial activation of procaspase-8. At the first cleavage step, the N-terminal p43/p41 and the C-terminal p30 cleavage products are generated. Importantly, these cleavage products already possess catalytic activity. At the second cleavage step, p43/p41 and p30 are processed to p10 and p20, respectively, which leads to the generation of the active caspase-8 heterotetramer (p20/p10)2.

UniProt: O89110

Pathways: Apoptose, Caspase Cascade in Apoptosis, Signalisation TLR, Activation of Innate immune Response, Tube Formation, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades